钙依赖性蛋白激酶Ⅱδ在脑外伤后皮质中的表达变化  

Expression of CaMKⅡδ in Cerebral Cortex Following Traumatic Brain Injury

作　　者：潘泓[1] 张静静[1] 徐冬冬[2] 谷振勇[1] 陶陆阳[3] 张明阳[1,3] 

机构地区：[1]南通大学医学院法医学系 [2]川北医学院法医学系 [3]苏州大学医学部法医学系

出　　处：《法医学杂志》2014年第3期169-171,177,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81301039);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：目的观察钙-钙调素依赖性蛋白激酶Ⅱδ(calcium-calmodulindependentproteinkinaseⅡδ,CaMKⅡδ)在脑损伤后皮质中不同时段的表达变化。方法建立大鼠脑外伤模型,通过Western印迹法和免疫组织化学染色法检测损伤区周围皮质CaMKⅡδ的表达变化。结果 Western印迹法显示,CaMKⅡδ在正常皮质中有表达,损伤后表达上调,在损伤3d达到高峰,并随着损伤时间延长逐渐恢复到正常水平。免疫组织化学结果与Western印迹法结果一致。结论 CaMKⅡδ在脑组织损伤灶及其周围呈现先增高后降低的表达变化,为法医学推断脑外伤后损伤经过时间提供了新的参考指标。Objective To observe the timecourse expression of calciumcalmodulin dependent protein kinaseⅡδ （CaMKⅡδ） in cerebral cortex after traumatic brain injury （TBI）. Methods The TBI rat model was established. The expression of CaMKⅡδ in cerebral cortex around injured area was tested by Western blotting and immunohistochemical staining . Results Western blotting revealed expression of CaMKⅡδ in normal rat brain cortex. It gradually increased after TBI, peaked after 3 days, and then returned to normal level. The result of immunohistochemical staining was consistent with that of Western blotting. Conclusion The expression of CaMKⅡδ around injured area after TBI increased initially and then decreased. It could be used as a new indicator for wound age determination following TBI.

关 键 词：法医病理学 脑损伤 钙-钙调素依赖性蛋白激酶Ⅱ 大鼠 

分 类 号：DF795.1[医药卫生—法医学]