IL-22和SOCS3在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎发病中的作用探讨  

Effect of IL-22 and SOCS3 in MHV-3 induced chronic viral hepatitis in mice

作　　者：李咏[1] 陆玉蕾[2] 吴婷[1] 韩梅芳[1] 宁琴[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染性疾病研究所/感染病科,武汉市430030 [2]广西医科大学附属肿瘤医院

出　　处：《实用肝脏病杂志》2014年第4期400-403,共4页Journal of Practical Hepatology

基　　金：国家自然科学基金项目(编号:NSFC81171558;NSFC81271808;NSFC81030007);教育部创新团队项目(编号:IRT1131)

摘　　要：目的探讨鼠3型肝炎病毒(MHV-3)诱导的慢性病毒性肝炎C3H/Hej小鼠肝组织白细胞介素-22(IL-22)和细胞因子信号抑制因子3(SOCS3)mRNA水平的变化。方法给C3H/Hej小鼠腹腔注射MHV-3(100pfu),诱导慢性病毒性肝炎模型,采用实时荧光定量PCR法检测MHV-3感染后0、5、10、15和20 d时小鼠肝组织IL-22和SOCS3 mRNA水平的变化。结果模型鼠肝组织IL-22和SOC3 mRNA水平在0 d时分别为(0.26±0.15)%和(6.35±2.21)%,在感染后10、15和20 d后IL-22水平分别为(6.28±2.79)%、(2.50±1.24)%和(2.73±0.85)%,SOC3水平分别为(16.92±4.39)%、(14.06±4.09)%和(13.36±1.89)%,均较0 d时显著增高(P<0.05),且在第10 d达到最高峰。结论 IL-22和SOCS3可能参与了MHV-3诱导的小鼠慢性病毒性肝炎的发生发展过程。Objective To investigate the changes of interleukin-22 （IL-22） and suppressor of cytokine signaling 3（SOCS3） mRNA in murine hepatitis virus 3（MHV-3） induced chronic viral hepatitis in C3H/Hej mice. Methods Chronic viral hepatitis in C3H/Hej mice was induced by intra-peritoneal injection of MHV-3 virus at dose of 100pfu. At day 0,5,10,15 and 20 post-infection,IL-22 and SOCS3 mRNA in the liver tissues were detected by real-time PCR. Results IL-22 and SOCS3 mRNA level in livers of model mice at day 0 were （0.26±0.15）%and（6.35±2.21）%,respectively. At day 10,15 and 20,IL-22 mRNA levels in the liver tissues were （6.28±2.79）%, （2.50±1.24）% and （2.73±0.85）%,respectively,and SOCS3 mRNA levels were （16.92±4.39）%,（14.06±4.09）% and （13.36±1.89）%,respectively,all of which were markedly increased than baseline （P﹤0.05）. The mRNA levels of IL-22 and SOCS3 both peaked at day 10 post MHV-3 infection. Conclusions IL-22 and SOCS3 may play an important role in the pathogenesis of MHV-3-induced chronic viral hepatitis in mice.

关 键 词：鼠3型肝炎病毒 慢性病毒性肝炎 白细胞介素-22 细胞因子信号抑制因子3 小鼠 

分 类 号：R512.6[医药卫生—内科学]