黄芪甲苷对高糖腹膜透析液诱导人腹膜间皮细胞表达致纤维化因子的影响  

Effect of Astragaloside Ⅳ on Expression of High Glucose Dialysis Solution-Induced Fibrosis Cytokines in Human Peritoneal Mesothelial Cells

作　　者：杨劲松[1] 李正红[1] 张旭[1] 盛梅笑[2] 

机构地区：[1]南京中医药大学研究生院,江苏南京210023 [2]南京中医药大学附属医院,江苏南京210029

出　　处：《中国中医药信息杂志》2014年第7期36-39,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：江苏省中医药领军人才项目(LJ200904);江苏省六大人才高峰项目(WS-033);江苏省高校优势学科建设工程资助项目(PAPD)

摘　　要：目的观察黄芪甲苷对高糖腹膜透析液(PDS)诱导人腹膜间皮细胞(HPMCs)结缔组织生长因子(CTGF)、转化生长因子-β1(TGF-β1)和血管内皮生长因子(VEGF)表达的影响,探讨黄芪甲苷对致纤维化因子的调节作用。方法选用HMrSV5细胞株,培养至第5代,随机分为对照组(10%FBS培养液)、模型组(4.25%PDS和10%FBS培养液)和黄芪甲苷低、中、高剂量组(含黄芪甲苷终浓度为10、20、40μg/mL的4.25%PDS和10%FBS培养液)。采用MTT法检测细胞活性,酶联免疫吸附法(ELISA)检测细胞上清液TGF-β1、CTGF、VEGF水平。结果除5μg/mL浓度组外,HPMCs的活性均较空白组不同程度增强,以40、45、50μg/mL浓度组作用较显著(P<0.05);与空白组比较,模型组TGF-β1、CTGF、VEGF表达增加;与模型组比较,黄芪甲苷各组TGF-β1、CTGF、VEGF表达明显减少,呈量效关系(P<0.05)。结论黄芪甲苷通过减少高糖刺激下HPMCs TGF-β1、CTGF、VEGF表达,达到延缓腹膜纤维化的发展。Objective To investigate the effects of AstragalosideⅣ （AST） on the expression of high glucose peritoneal dialysis solution （PDS）-induced transforming growth factor-β1 （TGF-β1）, connective tissue growth factor （CTGF）, vascular endothelial growth factor （VEGF） in cultured human peritoneal mesothelial cells. To discuss the regulating effect of AST on induced fibrosis cytokines.Methods The HMrSV5 cell line was cultivated to the fifth generation and divided into normal group （10%FBS cultivation solution）, model group （4.25% PDS and 10% FBS cultivation solution） and the low, medium and high doses AST groups （4.25% PDS with a respective 10, 20, 40 μg/mL AST）. MTT colorimetric assay was employed to detect cell activity and ELISA was applied to detect expression of TGF-β1, CTGF and VEGF in cultured supernatants.Results Except for 5 μg/mL group, HPMCs activity of high glucose plus different concentration AST groups were enhanced in different degrees, especially with 40, 45, and 50 μg/mL （P〈0.05）. The expression of TGF-β1, CTGF, and VEGF in model group increased. Compared with the control group, expression of the three AST groups significantly decreased and showed dose-effect relationship （P〈0.05）. Conclusion AST could reduce the expression of TGF-β1, CTGF and VEGF in high glucose-induced HPMCs and was useful in slowing down the progress of peritoneal fibrosis.

关 键 词：黄芪甲苷 人腹膜间皮细胞 转化生长因子-β1 结缔组织生长因子 血管内皮生长因子 腹膜纤维化 

分 类 号：R285.5[医药卫生—中药学]