回阳生肌膏及其拆方对糖尿病慢性皮肤溃疡大鼠血清白细胞介素-1α和血栓素B_2含量的影响  

Influence of Huiyang Shengji Ointment and Its Modified Formulae on Serum Interleukin-1α and Thromboxane B_2 in Diabetic Rats with Chronic Skin Ulcers

作　　者：李媛[1,2] 赵光明[2] 董建勋[1] 路广林[2] 张旭辉[2] 徐旭英[1] 魏然然[2] 陈铂[2] 

机构地区：[1]首都医科大学附属北京中医医院,北京100010 [2]北京中医药大学,北京100029

出　　处：《中国中医药信息杂志》2014年第7期43-46,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81072808);北京市教育委员会科技计划项目(KM201110025023);北京市高层次卫生技术人才学科带头人项目(2011-2-14)

摘　　要：目的观察回阳生肌膏及其拆方对糖尿病慢性皮肤溃疡大鼠白细胞介素-1α(IL-1α)和血栓素B2(TXB2)含量的影响,探讨其促进糖尿病慢性皮肤溃疡愈合的作用机制。方法 160只大鼠随机抽取6只作为空白组,不做任何处理,其余大鼠制备糖尿病模型,2周后随机分为1、3、5、7、14 d处理组,各处理组再分为正常组、模型组、回阳生肌膏组(全方组)、温阳益气拆方组(益气组)、活血生肌拆方组(活血组)。正常组及模型组给予凡士林纱条,全方组、益气组及活血组给予相应药物纱条。正常组采用皮肤缺损法,其他组采用复合因素制备慢性皮肤溃疡模型。在相应给药时间点处死各组大鼠,酶联免疫法检测各时点大鼠血清中IL-1α和TXB2的含量。结果 IL-1α含量:给药3 d益气组显著高于空白组、模型组、全方组和活血组(P<0.05);给药5 d,全方组显著高于空白组和模型组(P<0.05);给药7 d,各给药组显著高于空白组和模型组(P<0.05),全方组高于益气组、活血组(P<0.05);给药14 d,模型组和活血组低于空白组(P<0.05)。TXB2含量:给药3 d,正常组和全方组高于空白组(P<0.05);给药5 d,全方组高于空白组和正常组(P<0.05);给药7 d,益气组高于空白组、模型组、全方组和活血组(P<0.05);给药14 d,活血组高于空白组、模型组、全方组和益气组(P<0.05),空白组和正常组低于其他各给药组(P<0.05)。结论回阳生肌膏及其拆方均能促进炎症反应,刺激炎症因子分泌,益气温阳药对创面的促炎作用早期更为明显。Objective To observe the influence of Huiyang Shengji Ointment and its modified formulae on interleukin-1 （IL-1α） and thromboxane B2 （TXB2） in diabetic rats with chronic skin ulcers, and explore the mechanism for promoting the healing of ulcer.Methods Six out of 160 rats were randomly selected as a blank group, without any further processing. The remaining rats were made diabetic model and randomly divided into five groups after 2 weeks：1 d, 3 d, 5 d, 7 d and 14 d groups. Then, these groups were further divided into normal group （Vaseline ointments）, model group （Vaseline ointments）, Huiyang Shengji Ointment group （whole formula Ointment）, Wenyang Yiqi group （Yiqi group, modified Wenyang Yiqi formula ointments） and Huoxue Shengji group （Huoxue group, modified Huoxue Shengji formula ointments）. Normal group and model group were given Vaseline ointments;whole formula group, Yiqi group and Huoxue group were given corresponding ointment. Normal group used the method of skin excision, while other groups used STZ injection-hydrocortisone interference-skin excision-foreign body embedded preparation of composite factors for chronic skin ulcer model. After the appropriate treatment period, the rats were executed and tested for the contents of IL-1α and TXB2 in serum by enzyme-linked immunosorbent assay of five time points.Results In treatment 3 d, the contents of IL-1α in Yiqi group were significantly higher than the blank group, model group, whole formula group and Huoxue group （P〈0.05）. In treatment 5 d, the contents of IL-1α in whole formula group were significantly higher than the blank group and model group （P〈0.05）. In treatment 7 d, the contents of IL-1α in each treatment group were significantly higher than blank group and model group （P〈0.05）, and the whole formula group was higher than the Yiqi group and Huoxue group. In treatment 14 d, the contents of IL-1α in model group and Huoxue group were lower than the blank group （P〈0.05）. In treatment 3 d, the

关 键 词：回阳生肌膏 慢性皮肤溃疡 白细胞介素-1Α 血栓素B2 大鼠 

分 类 号：R285.5[医药卫生—中药学]