乙醇沉淀法与壳聚糖絮凝沉降法对疏郁复方颗粒中芍药苷含量的影响及纯化效果比较  

Purification Comparison and Influence of Ethanol Precipitation and Chitosan Flocculation Methods on Paeoniflorin Content in Shuyu Compound Granules

作　　者：高寒[1] 李淑琴[2] 马仁强 杨丽[1] 朱晓峰[4] 张荣华[1] 

机构地区：[1]暨南大学药学院,广东广州510632 [2]江门市药品检验所,广东江门529030 [3]广州博济医药生物技术股份有限公司,广东广州510602 [4]暨南大学第一附属医院,广东广州510630

出　　处：《中国中医药信息杂志》2014年第7期69-73,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：广州市科技计划(10A32070402)

摘　　要：目的比较乙醇沉淀法与壳聚糖絮凝沉降法对疏郁复方颗粒提取液的精制效果。方法采用正交试验法考察药液浓度、醇浓度、静置时间对乙醇沉淀法精制疏郁复方颗粒提取液的影响,并比较乙醇沉淀法与壳聚糖絮凝沉降法对固形物与芍药苷含量的影响。结果最佳的乙醇沉降工艺为:药液浓度0.8 g原药材/mL、醇浓度20%、静置6 h。乙醇沉淀法和壳聚糖絮凝沉降法均降低了疏郁复方颗粒煎煮液的出膏率,但壳聚糖絮凝沉降法对疏郁复方颗粒中芍药苷含量造成了较大损失。结论低浓度的醇沉工艺可以用于疏郁复方颗粒提取液的纯化,壳聚糖絮凝沉降法是否适用于该提取液的精制有待于进一步研究。Objective To compare the refining effects between ethanol precipitation and chitosan flocculation methods on extracting solutions of Shuyu compound granules.Methods Orthogonal experiment was utilized to examine the influence of liquid concentration, alcohol concentration and standing time on refining of Shuyu compound granules extracting solution by ethanol precipitation method, and compare the impact of ethanol precipitation and chitosan flocculation methods on the contents of solids and paeoniflorin.Results The optimized condition of ethanol precipitation method was 0.8 g/mL solution concentration （crude drug） with 20% ethanol standing for 6 h. Both ethanol precipitation method and chitosan flocculation method reduced the extraction ratio of Shuyu compound granules decoction, but chitosan flocculation method caused a great loss of paeoniflorin.Conclusion Low concentration ethanol precipitation method can be used for the purification of Shuyu compound granules. Whether chitosan flocculation method is suitable requires further study.

关 键 词：疏郁复方颗粒 壳聚糖絮凝沉降法 乙醇沉淀法 芍药苷 

分 类 号：R284.2[医药卫生—中药学]