RP-HPLC同时测定四逆散中4种有效成分含量  

Simultaneous Determination of Four Active Compounds in Sinisan by RP-HPLC

作　　者：李越峰[1] 吴平安[1] 王瑞琼[1] 宁艳梅[1] 黄玉钫 严兴科[1] 

机构地区：[1]甘肃中医学院甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000

出　　处：《中国中医药信息杂志》2014年第7期74-77,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81073077);教育部科学技术研究重点项目(212186);甘肃省省自然科学研究基金计划(1010RJZA212);甘肃省高等学校基本科研业务费项目(2013-2);甘肃中医学院引进人才科研启动基金(2010-3)

摘　　要：目的：建立同时测定四逆散中芍药苷、柚皮苷、橙皮苷、甘草酸的高效液相色谱方法,为科学评价及有效控制其质量提供可靠方法,并为四逆散的药效物质研究奠定基础。方法采用Kromasil C18柱（4.6 mm×250 mm,5μm）,流动相为乙腈-0.05%磷酸水溶液,梯度洗脱,检测波长为240 nm,流速1.0 mL/min,柱温30℃。结果芍药苷、柚皮苷、橙皮苷、甘草酸分别在0.00225～0.01125μg（r＝0.9997）、0.00390～0.00195μg （r＝0.9997）、0.00198～0.00099μg（r＝0.9995）、0.00262～0.00131μg（r＝0.9997）范围内线性关系良好,平均加样回收率分别为97.72%（RSD＝1.02%）、98.45%（RSD＝1.52%）、97.74%（RSD＝1.63%）、98.34%（RSD＝1.78%）。结论所建立的方法简单、准确、可靠,重复性好,可用于四逆散的质量控制。Objective To build a method to simultaneously determine paeoniflorin, naringin, hesperidin, glycyrrhizic acid in Sinisan by HPLC, provide a reliable method for evaluation and effective quality control, and lay the foundation of material basis for efficacy research for TCM compound Sinisan.Methods The column was Kromasil C18 （4.6 mm×250 mm, 5μm）;mobile phase was acetonitrile-0.05% phosphoric acid aqueous solution with gradient elution program;the flow rate was 1.0 mL/min;UV detection was performed at 240 nm;the detector drift tube temperature was set at 30℃.Results The linear ranges of paeoniflorin, naringin, hesperidin and glycyrrhizic acid were 0.00225-0.01125μg （r=0.999 7）, 0.00390-0.00195μg （r=0.9997）, 0.00198-0.00099μg （r=0.999 5）, 0.00262-0.00131μg （r=0.999 7）, respectively. The average recoveries of paeoniflorin, hesperidin, naringin and glycyrrhizic acid were 97.72% （RSD=1.02%）, 98.45% （RSD=1.52%）, 97.74% （RSD=1.63%）, 98.34% （RSD=1.78%）, respectively.Conclusion The method is accurate, simple, reliable and duplicable, and is available for the quality control of Sinisan.

关 键 词：四逆散 芍药苷 柚皮苷 橙皮苷 甘草酸 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]