大肠埃希菌和肺炎克雷伯菌质粒β-内酰胺酶的筛选  

The screen of plasmid mediated β-lactamases in Escherichia coli and Klebsiella pneumoniae

作　　者：蒋燕群[1] 王坚强[1] 陈珊珊[1] 李桂兰[1] 倪语星[2] 

机构地区：[1]上海市第六人民医院,上海200233 [2]上海第二医科大学端金医院,上海200025

出　　处：《上海医学检验杂志》2001年第1期10-12,共3页Shanghai Journal of Medical Laboratory Sciences

摘　　要：目的了解大肠埃希菌和肺炎克雷伯菌的质粒介导的 β-内酰胺酶的分布、表型及其检测方法。方法经头孢泊肟初筛 ,分别用 NCCL S确证试验检测超广谱 β-内酰胺酶 (ESBL)和用头孢西丁三相试验检测质粒 Am p C。结果 34 1株大肠埃希菌和肺炎克雷伯菌中经常规药敏试验筛选出 16 4株 (48.1% )头孢泊肟 (CPD)的 MIC>1μg/ m l,对其中的 10 2株进行检测 ,结果产 ESBL 的有 6 4株 ,头孢西丁三相试验阳性者有 6株。结论大肠埃希菌和肺炎克雷伯菌的质粒 β-内酰胺酶以 ESBL 为主 (30 .2 % ) ,质粒 Amp Cs仅占 2 .8%。Objective To study distribution,phenotypes and detection methods of plasmid mediated β lactamases in Escherichia coli and Klebsiella pneumoniae.Methods Using routine antibiotics susceptibility tests for screening,extended spectrum β lactamases (ESBL) confirm test for detecting ESBL,and cefoxitin three dimension test for detecting plasmid mediated AmpCs.Result Among 341 clinical isolates of Escherichia coli and Klebsiella pneumoniae,there are 164 strains (48 1%) showed MIC of cefpodoxime>1 μg/ml by routine susceptibility test;102 strains of them were proceeded further detection,64 strains produced ESBL,only 6 strains were plasmidincode AmpCs.Conclusion The result indicated that ESBL (30 2%) were more than plasmid incode AmpCs (2 8%) in plasmid mediated β lactamases in Escherichia coli and Klebsiella pneumoniae.

关 键 词：超广谱Β-内酰胺酶 质粒AmpC 大肠埃希菌 肺炎克雷伯菌 

分 类 号：Q556.4[生物学—生物化学]