母乳中烷氧基甘油含量的测定  

Determination of alkylglycerol contents in breast milk

作　　者：钱林溪[1,2] 宋还雷[1,2] 郑涛[3] 钟燕[4] 喻文娟 吴圣楣[1] 蔡威[1,2] 

机构地区：[1]上海交通大学医学院附属新华医院上海市儿科医学研究所,上海200092 [2]上海市小儿消化营养重点实验室,上海200092 [3]上海交通大学医学院附属新华医院妇产科,上海200092 [4]Global Research & Discovery Mead Johnson Nutrition [5]上海交通大学分析测试中心,上海200240

出　　处：《临床儿科杂志》2014年第6期540-543,共4页Journal of Clinical Pediatrics

基　　金：美赞臣基础研发基金

摘　　要：目的探讨哺乳期妇女乳汁中烷氧基甘油(AKGs)含量及其变化规律。方法选取2011年6月至2012年6月足月单胎分娩的健康产妇5例,收集哺乳期第1、2、3、4、8、12、16、20、24周母乳用于AKGs测定。母乳样品经萃取、皂化、提取、衍生化处理后,通过气质联用色谱内标法对母乳中AKGs成分进行定量分析。结果母乳中16C∶0 AKG含量逐渐降低,均值从(17.31±3.59)×10-3g/L降低到(11.14±1.83)×10-3g/L,而18C∶0 AKG均值从(14.95±6.00)×10-3g/L下降到(9.68±2.51)×10-3g/L,18C∶1 AKG均值在(4.64±0.91)×10-3g/L到(3.95±0.68)×10-3g/L之间。结论通过气质联用色谱内标法检测到哺乳妇女母乳中存在16C∶0、18C∶0和18C∶1三种AKG,其浓度在哺乳期不同阶段有所改变。Objective To determine alkylglycerol （AKG） contents and variation in breast-milk of lactating women. Methods Five cases of healthy lactating women with term delivery were selected from June 2011 to June 2012. Breast-milk samples were collected at 1, 2, 3, 4, 8, 12, 16, 20 and 24 weeks postpartum. Breast milk samples were extracted, saponificated and derivatized. AKGs composition in breast-milk was quantitatively analyzed by GC chromatography. Results Mean 16C：0 AKG content in breast-milk decreased from（17.31 ± 3.59）× 10-3g/L to（11.14 ± 1.83）× 10-3g/L. Mean 18C：0 AKG content decreased from（14.95±6.00）×10-3g/L to（9.68±2.51）×10-3g/L. Mean 18C：1 AKG content fluctuated between（4.64±0.91）×10-3g/L and（3.95±0.68）×10-3g/L. Conclusions 16C：0, 18C：0 and 18C：1 AKG contents exist in Chinese breast-milk through determination by GC chromatography, and the concentrations vary among different stages of lactation.

关 键 词：母乳 烷氧基甘油 气相色谱 

分 类 号：R927[医药卫生—药学] O657.71[理学—分析化学]