血叶兰的组织培养与快速繁殖  

Tissue Culture and Rapid Propagation of Ludisia discolor

作　　者：朱桥[1] 丁俊伟 杨学文[1] 梁廉[1] 邵玲[1] 

机构地区：[1]肇庆学院生命科学学院,广东肇庆526061

出　　处：《植物生理学报》2014年第6期805-809,共5页Plant Physiology Journal

基　　金：广东省大学生创新项目(1058012034);肇庆市科技计划项目(2013N007)

摘　　要：以血叶兰的嫩茎段为外植体,建立了血叶兰的组培快繁体系。结果表明:芽诱导最适宜的培养基是MS+6-BA 5.0mg·L-1;最适芽增殖培养基为MS+6-BA 5.0 mg·L-1+NAA 0.5 mg·L-1+TDZ 0.3 mg·L-1,芽增殖率达4.92倍;最佳壮苗培养基为MS+NAA 0.3 mg·L-1+GA3 1.0 mg·L-1+15%椰汁,芽苗高度达到4.33 cm,芽粗为0.61 cm;生根最适培养基为1/2MS+IBA 1.0mg·L-1+15%香蕉+0.5 g·L-1活性炭,生根率在93.0%以上;炼苗后,移栽在泥炭土+珍珠岩+松树皮(3:1:1,V/V/V)混合基质中,存活率高于87.0%。This paper deals with tissue culture of Ludisia discolor by tender stem explants. The results showed the suitable medium of initial was MS medium supplemented with 5.0 mg·L-1 6-BA. The best proliferation occurred on MS medium supplemented with 5.0 mg·L-1 6-BA and 0.5 mg·L-1 NAA and 0.3 mg·L-1 TDZ, the propagation coefficient of adventitious shoots reached 4.92. The best hardening medium was MS medium supplemented with 0.3 mg·L-1 NAA and 1.0 mg·L-1 GA3 and 15% coconut milk, with the average plantlet height of 4.33 cm and the average stem diameter of 0.61 cm. The best rooting for plantlets occurred on 1/2MS medium supplemented with 1.0 mg·LI IBA and 15% banana and 0.5 g·L-1 acfivated carbons, with rooting rate up to 93.0%. The plantlets with acclimatization were transferred to the the mixed matrix [peat soil：vermiculite：pine bark=3：l·L （V/V/V）] and the outplanting survival rate was 87.0%.

关 键 词：血叶兰 组织培养 快速繁殖 

分 类 号：S682.31[农业科学—观赏园艺]