针刺“长强”穴对自闭症模型大鼠学习记忆能力和前额叶皮层缝隙连接相关蛋白表达的影响  

Influence of Acupuncture of “Changqiang”(GV 1) on Learning-memory Ability and Gap Junction-related Protein Expression in the Prefrontal Cortex in Autism Rats

作　　者：洪钰竺[1] 张学君[1] 洪霖[1] 黄倩茹[1] 吴强[1] 

机构地区：[1]福建中医药大学针灸学院,福州350122

出　　处：《针刺研究》2014年第3期173-179,共7页Acupuncture Research

基　　金：国家自然科学基金资助(No.81202764);福建省教育厅资助(No.JK 2012022);福建省科技厅资助(No.2012Y0045)

摘　　要：目的:观察针刺"长强"穴对自闭症模型大鼠学习和记忆能力及对前额叶皮层缝隙连接相关蛋白(CX)表达的影响,探讨针刺"长强"穴改善自闭症大鼠学习记忆能力的可能机制。方法:Wistar孕鼠予以腹腔注射丙戊酸钠后所产子代,经睁眼试验、游泳试验筛选后,确定为自闭症大鼠模型,随机挑选30只,分为长强组、非穴组、模型组,每组10只,正常Wistar大鼠子代随机挑选10只作为空白组。长强组针刺"长强",每次1min,10d为1个疗程,干预3个疗程;非穴组取胁下非经非穴点针刺。采用Morris水迷宫观察大鼠学习记忆能力,免疫组化法检测大鼠前额叶皮层CX 43、CX 32、CX 36蛋白的表达。结果:自闭症模型组幼鼠睁眼时间在出生后14d(PN 14)、PN 15、PN 16较空白组晚(P<0.05),游泳能力在PN 13和PN 15时较空白组低下(P<0.05)。治疗前长强组、非穴组、模型组逃避潜伏期及平均速度与空白组比较,差异均有统计学意义(P<0.05);治疗后长强组较模型组及非穴组明显改善(P<0.05)。与空白组比较,模型组CX 43、CX32、CX 36蛋白的表达显著降低(P<0.05);治疗后,长强组CX 43、CX 32、CX 36蛋白的表达显著高于模型组和非穴组(P<0.05)。结论:针刺"长强"穴能通过提高前额叶皮层CX 43、CX 32、CX 36蛋白的表达来改善自闭症模型大鼠学习和记忆能力。Objective To observe the effect of acupuncture stimulation of ＂Changqiang＂ （GV 1） on learning-memory ability and gap junction-related protein expression in the prefrontal cortex in autism rats. Methods Forty Wistar rats were equally randomized into control, model, GV 1 and non-acupoint groups. For establishing autism model, Valproate acid （VPA） sodium （600 mg/kg） was given （i. p. ） to pregnancy rats whose intimate filial generation was confirmed to be successful autism by eyeopen tests, swimming test and Morris water maze swimming tasks. GV 1 or non-acupoint （the spot below the costal region, i.e., 2 cm superior to the posterior superior lilac spine and about 3 cm lateral to the spine） was punctured and stimulated for about 1 min by using a filiform needle, once daily for 30 days except the weekends. The rats＇ learning-memory ability was detected by Morris water maze tasks. The expression of gap junction-related proteins connexin 43 （CX 43）, CX 32 and CX 36 in the frontal cortex tissue was detected by immunohistochemistry. Results After modeling, the postnatal rats＇ eyeopen time on day 14, 15 and 16 was significantly later （P〈0.05）; and the swimming ability on postnatal day 13 and 15 was obviously lower in comparison with that of the control group （P〈0.05）. After acupuncture treatment, the increased escape latency and the decreased swimming velocity of the autism rats were obviously suppressed in the GV 1 group, rather than in the non-acupoint group （P〈0. 05）. It suggests an improvement of learning-memory ability after acupuncture stimulation of GV 1. In comparison with the control group, the expression levels of cerebral CX 43, CX 32 and CX 36 proteins （mean grey values） were considerably down-regulated in the model group （ P〈0. 05）. While compared to the model group, their expression levels were apparently up-regulated in the GV 1 group （P〈0.05） but not in the non-acupoint group. Conclusion Acupuncture intervention of GV 1 can improve the learnin

关 键 词：自闭症 针刺干预 长强穴 缝隙连接蛋白 CX 43 32 36 学习记忆能力 

分 类 号：R245.311[医药卫生—针灸推拿学]