电针“关元”穴对去卵巢骨质疏松症大鼠血清胰岛素样生长因子-1和骨生物力学的影响  

Effects of Electroacupuncture Stimulation at “Guanyuan”(CV 4) on Serum Insulin-like Growth Factor-1 Content and Bone Biomechanics in Ovariectomy-induced Osteoporosis Rats

作　　者：张木兰[1] 李沛[2] 林莺[2] 纪峰[1] 

机构地区：[1]福建中医药大学针灸学院,福州350122 [2]福建中医药大学海外教育学院,福州350003

出　　处：《针刺研究》2014年第3期207-210,221,共5页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.81173347/H 2718);国家自然科学基金青年科学基金项目(No.81102651/H 2718)

摘　　要：目的:探讨针刺治疗去卵巢骨质疏松症的作用机制。方法:4.5月龄SD雌性大鼠40只,随机分为假手术组、模型组、西药组和电针组。切除大鼠双侧卵巢建立绝经后骨质疏松症模型。电针组电针刺激"关元"穴20min,每日1次,10次为1个疗程,共3个疗程;西药组大鼠戊酸雌二醇片生理盐水混悬液(25μg/mL,2mL/500g体重)灌胃,疗程同电针组。采用ELISA法检测大鼠血清胰岛素样生长因子-1(IGF-1)水平,运用三点弯曲试验检测大鼠股骨骨生物力学的变化。结果:与假手术组相比,模型组大鼠血清IGF-1,股骨最大载荷、断裂载荷均显著性降低(P<0.05,P<0.01);与模型组相比,电针组、西药组大鼠血清IGF-1,股骨最大载荷、断裂载荷均显著性升高(P<0.05,P<0.01);电针组与西药组相比,差异无统计学意义(P>0.05)。结论:电针通过提高大鼠血清IGF-1水平和骨强度,发挥对绝经后骨质疏松症的治疗作用。Objective To observe the effect of electroacupuncture （EA） on serum insulin-like growth factor-1 （IGF-1） content and bone biomechanics in osteoporosis rats so as to explore its mechanism underlying improvement of postmenopausal osteoporosis （PMOP）. Methods Forty female SD rats （4.5 months old） were randomly divided into sham-operation （sham）, model, medication and EA groups （10 rats/group）. The rat＇s bilateral ovaries were removed to establish the PMOP model. For rats in the sham group, similar procedures were conducted except removing comparable weight of fat tissues around the ovaries. For rats in the EA group, ＂Guanyuan＂ （CV 4） was punctured with filiform needles and stimulated electrically （1 mA, 2 Hz） for 20 min, once a day for 30 days. For rats in the medication group, pentanoic acid estradiol （50 μg/500 g） was administrated by gavage. The serum IGF-1 content was examined by ELISA and the bone biomechanics measured by three-point bending tests, respectively. Results Compared with the sham group, the serum IGF-1 level, femoral maximum load and fracture load were significantly reduced in the model group （P〈0.05, P〈0.01 ）. In comparison with the model group, these indexes were significantly increased in rats of the EA and medication groups （P〈0.05, P〈0. 01）, but without significant difference between the EA and medication groups （P〈0.05）. Conclusion EA produces benefits on postmenopausal osteoporosis through increasing the serum IGF-1 contents and bone strength.

关 键 词：绝经后骨质疏松症 电针干预 胰岛素样生长因子-1 骨生物力学 

分 类 号：R245.97[医药卫生—针灸推拿学]