直接灸“肝俞”对原发性肝癌癌前病变大鼠凋亡抑制因子Livin的影响  

Effects of Moxibustion at “Ganshu”(BL 18) on Serum Alpha-fatoprotein and Liver Livin Levels in Rats with Precancerous Lesion of Primary Hepatocellular Carcinoma

作　　者：贾文睿 董峰[2] 谢锡亮 王培[1] 张秋菊[1] 王玥琦[1] 侯中伟[1] 睢明河[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]中国中医科学院针灸医院,北京100700 [3]山西省澄江学派针灸医学研究所,侯马043000

出　　处：《针刺研究》2014年第3期211-215,共5页Acupuncture Research

基　　金：教育部博士点基金项目(20100013120014)

摘　　要：目的:观察直接灸"肝俞"对原发性肝癌(HCC)癌前病变大鼠凋亡抑制因子Livin的影响,探讨直接灸法对HCC癌前病变大鼠肝癌变的延缓作用及其机制。方法:将60只Wistar大鼠分为正常组(10只)、模型组(20只)、预防1组(15只)、预防2组(15只)。以二乙基亚硝胺逐步诱导建立HCC模型,为期12周。预防1组、预防2组从造模开始即给予"肝俞"穴麦粒灸,5壮/穴,0.5mg/壮,隔日1次,分别持续一疗程(20d)与两疗程(40d),预防结束后继续造模至第12周。ELISA法检测血清甲胎蛋白(AFP)含量变化,免疫组化法检测凋亡抑制因子Livin在肝脏组织中的表达情况。结果:预防第1疗程结束时,与正常组相比,模型组AFP含量显著升高(P<0.01),预防1组AFP含量较模型组显著降低(P<0.05);预防第2疗程结束时,模型组AFP含量较正常组显著升高(P<0.05),预防2组AFP含量与模型组比较有降低趋势,但差异无统计学意义(P>0.05);造模结束时,与正常组相比,模型组AFP含量显著升高(P<0.01)。造模结束时Livin在模型组肝脏组织中表达的累积吸光度(IOD)值与正常组相比显著升高(P<0.01);预防1组、预防2组与模型组相比,Livin表达的IOD值均显著降低(P<0.01,P<0.05)。结论:直接灸法通过增强肝细胞自我修复功能、降低凋亡抑制因子Livin的表达,从而抑制肝癌细胞生长,促进其凋亡,可能对延缓HCC癌前病变发挥一定的作用。Objective To observe the effect of moxibustion at ＂Ganshu＂ （BL18） on levels of Livin （a novel member of the inhibitors of apoptosis family and plays crucial roles in apoptosis, cell proliferation, and cell cycle control） in the liver tissue in rats with precancerous lesion of primary hepatocellular carcinoma （HOG）, so as to explore its mechanism underlying improvement of HCC. Methods Sixty male Wistar rats were randomly divided into control group （n = 10）, model group （n = 20）, moxibustion-20 d group （n = 15） and moxibustion-40 d group （n = 15）. HCC model was established by intraperitoneal injection of dieth- ylnitrosamine （DEN, 50 mg/kg）, once every 3 days for 12 weeks. Moxibustion was applied to bilateral ＂Ganshu＂ （BL 18）, once every other day for 10 times （moxibustion-20 d group） and 20 times （moxibustion-40 d group）, respectively. Serum alpha-fetoprotein （AFP） content was detected with ELISA and Livin immunoactivity of the liver tissue detected using immunohistochemistry. Results After modeling, serum AFP content and liver Livin immunoactivity of the model group were significantly increased in comparison with the control group（P〈0.01）. After moxibustion treatment, serum AFP content in the moxibustion-20 d group and liver Livin immunoactivity in both moxibustion-20 d and moxibustion-40 d groups were remarkably down-regulated in comparison with the model group（ P^0.05）. No significant differences were found between the moxibustion-40 d group and the model group in serum AFP content （P〈0.05）. ConclusJen Moxibustion can down-regulate liver Livin protein expression, which probably has a role in promoting hepatocellular apoptosis to inhibit precancerous lesion and to postpone hepatocarcinogenesis.

关 键 词：艾灸 原发性肝细胞肝癌 癌前病变 凋亡抑制 肝脏Livin 甲胎蛋白 

分 类 号：R245.81[医药卫生—针灸推拿学]