茜草对大鼠股骨显微结构的影响  

Effect of madder on bone micro-structure in rats

作　　者：吴晨晨[1] 杨小雯[1] 王文龙[1] 王姗姗[1] 曹丹丹[1] 马烽[1] 王建国[1] 路浩[1] 赵宝玉[1] 

机构地区：[1]西北农林科技大学动物医学院,中国陕西省杨陵示范区712100

出　　处：《中国骨质疏松杂志》2014年第6期658-661,共4页Chinese Journal of Osteoporosis

基　　金：国家自然基金-青年项目(31302153);校博士启动金(Z111021305)

摘　　要：目的茜草对骨骼具有红染的效果,探索茜草如何影响骨骼的显微结构,是否饲喂茜草对大鼠的内脏器官具有显著的毒害作用。方法本试验选取80只1月龄大鼠,随机分为4组(分别添加茜草量为0%、5%、10%和15%入日粮中),饲喂后0.5、1、3和6个月,采取双侧股骨、内脏和血液。结果饲喂茜草6个月后,10%和15%组大鼠股骨骨干的平均厚度、骨干内径、骨干外径、骨髓腔面积、皮质骨面积、骨干总面积和股骨骨干的骨矿物质密度显著高于0%组大鼠;股骨远端骨小梁体积百分比,骨小梁厚度,连接密度,骨小梁数量和股骨远端的骨矿物质密度的水平均显著高于0%组大鼠,而股骨远端的骨小梁分离度和结构模型指数显著低于0%组大鼠。但是饲喂茜草6个月后,15%组的大鼠血液中尿素氮和肌酐的含量与0%组大鼠之间差异显著。结论饲喂10%茜草对大鼠无毒性影响,并能促进大鼠的骨骼长度和骨的显微结构,为以后茜草的合理应用提供了实验基础。Objective Bone could be stained with red color by madder.However, whether feeding with the madder has significantly toxicity in animals was still unknown.This study was designed to explore this question.Methods Eighty 1-month rats were selected in this study, and then randomly divided into 6 groups （0%, 5%, 10%, or 15%madder added in diet）.After 0.5-, 1-, 3-, and 6-month feeding, all the rats were executed.And the bilateral femur, the internal organs, and the blood were collected.Results The results showed that the mean thickness, the inner diameter, the external diameter, the marrow cavity area, the cortical bone area, the total area, and the bone mineral density of the femur diaphysis in 10% group and 15%group increased significantly compared with those in 0%group.The trabecular bone volume, the trabecular thickness, the connectivity density, the trabecular number, and the bone mineral density of the distal femur in 10% group were higher than those in 0% group.The trabecular separation and the structure model index of the distal femur in 10%group were lower than those in 0%group.However, after 6-month feeding, the blood levels of urea nitrogen and creatinine in 15% group were significantly different with that in 0%group.Conclusion There is no toxicity of 10%madder in the diet for rats.Madder can improve the length of the femur and the bone micro-structure, which provides the experimental basis for the reasonable application of madder.

关 键 词：茜草 大鼠 骨显微结构 股骨 

分 类 号：Q955[生物学—动物学]