Conversion of female germline stem cells from neonatal and prepubertal mice into pluripotent stern cells  

作　　者：Hu Wang Manxi Jiang Haiwei Bi Xuejin Chen Lin He Xiaoyong Li Ji Wu 

机构地区：[1]Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, China [2]Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

出　　处：《Journal of Molecular Cell Biology》2014年第2期164-171,共8页分子细胞生物学报（英文版）

基　　金：Funding This work was supported by National Basic Research Program of China （2013CB967401 and 2010CB945001）, and the National Nature Science Foundation of China （81370675 and 81121001）.

摘　　要：Pluripotent stem cells derived from neonatal or adult testes are a useful tool to examine the mechanisms of pluripotency and a resource for cell-based therapies. However, therapies usingthese cells will only benefit males but not females. Recently, female germline stem cells （FGSCs） were discovered in ovaries. Whether FGSCs can be converted into pluripotent stem cells, similar to spermatogonial stem cells, is unknown. Here, we demonstrate that female embryonic stem-like cells （fESLCs） can be generated within 1 month from the stably proliferating FGSCs cultured in embryonic stem cell （ESC） medium, fESLCs exhibit properties similar to those of ESCs in terms of marker expression and differentiation potential. Thus, our findings suggest that generation of patient-specific fESLCs is feasible and provides a foundation for personalized regenerative applications.

关 键 词：embryonic stem-like cells female germline stem cells adult stem cells 

分 类 号：Q581[生物学—生物化学] N1[自然科学总论]