莪术醇软膏对糖尿病溃疡创面愈合的作用  

Research on accelerated healing of skin wounds of diabetic ulceration by curcumol ointment

作　　者：周洁[1] 朱雁林[1] 董建勇[1] 傅红兴[1] 裘关关[1] 方亮莲[1] 林潍潍 

机构地区：[1]温州医科大学药学院,浙江温州325035

出　　处：《温州医学院学报》2014年第6期411-416,共6页Journal of Wenzhou Medical College

基　　金：浙江省大学生科技创新活动计划(2012R413045)

摘　　要：目的:研究莪术醇软膏对糖尿病溃疡愈合的治疗作用。方法:SD大鼠腹腔注射链脲佐菌素(STZ,55 mg/kg)建立糖尿病模型,1周后测定血糖,血糖值大于16.7 mmol/L为造模成功。2周后,在大鼠背部脊柱两侧用手术剪各剪切2个直径为1.8 cm的圆形全层皮肤,糖尿病溃疡造模成功后,随机将大鼠分为3组:空白基质组、5%莪术醇软膏组和10%莪术醇软膏组。给药后于第3、第7、第14和第21天对溃疡愈合率、组织形态学进行观察与检测。结果:实验造模成功后,莪术醇软膏组的创面愈合明显比空白基质组快(P<0.05),且高剂量组创面愈合较低剂量组愈合更佳。病理切片HE染色及免疫组织化学染色的结果表明3组中10%莪术醇软膏组在促进创面愈合,加快胶原合成,增加内皮细胞黏附分子(CD31)的阳性表达,上调转化生长因子-β1(TGF-β1)蛋白阳性表达上效果最好。结论:莪术醇软膏可促进糖尿病溃疡的愈合,为临床使用外用药治疗糖尿病性溃疡提供实验参考。Objective： To study the effect of topical administration of curcumol ointment on the wound healing process in diabetic rats.Methods： The study was conducted using male Sprague Dawley rats which were separated into three groups： diabetic rats receiving control cream, diabetic rats receiving 5% curcumol ointment, and diabetic rats receiving 10% curcumol ointment. Two 1.8 cm diameter cutaneous wounds were then created at the dorsal regions of each rats. Wound healing was assessed by rate of wound closure estimation, and histologi-cal studies on the 3, 7, 14 and 21 d after wounding.Results： Curcumol ointment treatment enhanced the wound healing process by increasing rate of wound closure signiifcantly compared to the control group （P〈0.05）. His-tological observation also showed the 10% curcumol ointment treated groups signiifcantly promoted the number of capillaries, improved the collagen synthesis, and increased the positive expressions of CD31 and TGF-β1. Conclusion： Curcumol ointment administrations can improve the speed and quality of skin wound healing.which could provide theoretical foundation for clinical treatment of diabetic ulcer.

关 键 词：糖尿病溃疡 莪术醇 软膏 胶原三色染色法 CD31 转化生长因子-Β1 大鼠 

分 类 号：R961.1[医药卫生—药理学]