小麦籽粒干物质积累的动态QTL定位  

Dynamic QTL Analysis for Kernel Dry Matter Accumulation in Wheat

作　　者：李美霞[1] 沈玮囡[1] 杨睿[1] 梁子英 孙风丽[1] 奚亚军[1] 王竹林[1] 刘曙东[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨陵712100

出　　处：《西北植物学报》2014年第6期1105-1111,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家高技术研究发展计划(863)项目(2011AA100501);西北农林科技大学唐仲英植物育种基金;陕西省农业厅小麦种质资源保护项目

摘　　要：以波兰小麦品系‘XN555’与普通小麦品系‘中13’杂交产生的99个F10重组自交系(RILs)为材料,构建了包含241个SSR分子标记的A、B染色体组14个连锁群的遗传图谱,并采用Logistic方程拟合籽粒灌浆过程,对粒重增长的缓慢增长期、快速增长期和平稳期进行千粒重条件QTL和非条件QTL定位分析。结果显示:(1)在小麦A、B染色体组上共检测到5个非条件QTL和5个条件QTL。(2)在小麦粒重缓慢增长期和快速增长期各有2个非条件QTL,平稳期有1个非条件QTL,它们分别位于2B、3A、3B和7A染色体上,单个QTL可解释表型变异的9.66%～15.18%。(3)在小麦粒重快速增长期检测到1个条件QTL,平稳期检测到4个条件QTL,涉及1A、2B、5B和7B染色体,单个QTL可解释表型变异的13.01%～29.27%。(4)于2B染色体Xbarc361～Xwmc422标记区间距Xbarc361标记0.05cM处,在粒重快速增长期同时检测到一个条件QTL和非条件QTL,且在平稳期检测到一个非条件QTL。研究表明,小麦不同灌浆时期粒重增长相关QTL的数量和遗传效应各不同,同一QTL在不同灌浆时期的遗传效应也不同,即QTL的表达具有时序选择性。A genetic map composed of 14 linkage groups including A genome and B genome was constructed with 241 SSR（Simple Sequence Repeat） markers by using a population consisted of 99 F10 recombinant inbred lines（RILs） derived from a cross of Triticum polonicum L.line ‘XN555’ and Triticum aestivum L.line ‘Zhong 13’.With growth curve fitting of Logistic equation,conditional and unconditional QTLs for thousand-grain weight in the period of slow growth,rapid growth and plateau phase were detected.（1）5 unconditional QTLs and 5 conditional QTLs for thousand-grain weight were detected on A genome and B genome in total.（2）Of unconditional QTLs,two QTLs were found in the period of slow growth and rapid growth each and one QTL was found in the plateau phase,associating with chromosomes 2B,3A,3B and 7A.The phenotypic contribution of individual unconditional QTL was in the range of 9.66%~15.18%.（3）Of conditional QTLs,one QTL was detected in the period of rapid growth and four were detected in the plateau phase,involving chromosomes 1A,2B,5B and 7B,respectively.The phenotypic contribution of individual conditional QTL ranged from 13.01% to 29.27%.（4）One unconditional QTL and one conditional QTL in the period of rapid growth,as along with one unconditional QTL in the plateau phase,were detected between markers Xbarc361 and Xwmc422 with a distance of 0.05 cM away from marker Xbarc361 on chromosome 2B.For dry matter accumulation of wheat kernels,numbers of QTLs and the genetic effects of the same QTL were different in differing grain filling periods,which indicated time-dependent and sequenced expression of QTLs.

关 键 词：小麦 干物质积累 粒重 动态QTL 

分 类 号：Q343.17[生物学—遗传学]