蛋鸡卵巢PRSS23基因CDS序列分析  

Analysis on the Complete CDS Sequence of PRSS23 in Hen Ovaries

作　　者：孟金柱[1] 李鹏飞[2] 姜小龙[1] 陈建伟[1] 刘岩[1] 黄洋[1] 吕丽华 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]山西农业大学生命科学学院,太谷030801

出　　处：《基因组学与应用生物学》2014年第1期77-81,共5页Genomics and Applied Biology

基　　金：国家自然科学基金(31172211);山西省引进人才基金项目(2008080);山西省国际科技合作项目(2010081002);国家重点实验室开放课题(SNKF-2012-02);山西农业大学横向协作与委托项目(2010HX54)共同资助

摘　　要：为获得蛋鸡丝氨酸蛋白酶23(PRSS23)的CDS序列并分析其结构域,试验根据NCBI中公布的其它物种PRSS23的mRNA序列,设计了特异性引物,采用RT-PCR技术扩增PRSS23 CDS全序列。BLAST比对发现,与其它物种PRSS23预测序列以及氨基酸序列同源性分别为99%、92%、91%、86%和100%、96%、96%、90%;通过分析该序列的三级结构,发现在第117到362位氨基酸之间含有一个典型的Tyrp-SPc结构域,该结构域具有典型丝氨酸肽链内切酶活性;通过对其蛋白信号肽预测分析,发现序列中含有信号肽,信号锚定的可能性为72.8%。To obtain and analysis the complete CDS sequence of hen ovarian PRSS23 （serine protease 23）, specific primers were designed according to the PRSS23 mRNA sequence of other species in NCBI and RT-PCR techonlogy was performed. The results showed that the nucleotide sequence and the deduced amino acid sequence of hen ovary PRSS23 shared 99%, 92%, 91%, 86% and 100%, 96%, 96%, 90% identity with that of other selected species, respectively. And we found PRSS23 contained a typical Tyrp-SPc domain among 117-362 amino acids by the tertiary structure analysis, which had a typical serine endopeptidase activity. Signal peptide was predicted and found that there contained signal peptide in the sequence and the signal anchor probability was 72.8%.

关 键 词：蛋鸡 PRSS23 卵泡 序列分析 

分 类 号：S831[农业科学—畜牧学]