FGF1a(fibroblast growth factor 1a)基因对镜鲤(Mirror carp)鳞被发育的影响  

Effect of FGF1a(fibroblast growth factor 1a) Gene on Mirror Carp Scale Development

作　　者：马龙[1,2] 蒋丽[2] 王阳阳[2,3] 程安达[2,4] 乔志刚[1] 李恒德[2] 

机构地区：[1]河南师范大学水产学院,新乡453007 [2]中国水产科学研究院生物技术研究中心,北京100141 [3]大连海洋大学生命科学与技术学院,大连116023 [4]上海海洋大学水产与生命学院,上海201306

出　　处：《基因组学与应用生物学》2014年第1期82-87,共6页Genomics and Applied Biology

摘　　要：成纤维生长因子FGF1a(fibroblast growth factor 1a)是一类重要的生长因子,在细胞的发育过程中起着重要的作用。用实验室镜鲤EST数据库设计包含该基因CDS全长的特异引物,得到了FGF1a基因的全长CDS。经过克隆测序发现,镜鲤FGF1a基因全长的CDS包含444个碱基,其编码一个含有146个氨基酸的蛋白质,包含一个受体结合位点。与其它物种序列比对结果表明,镜鲤FGF1a蛋白与斑马鱼相似度最高,而与其亲缘关系较远的人类、老鼠、非洲爪蟾蜍及鸡相似度较低。根据所获得的基因序列设计探针,利用原位杂交技术发现FGF1a基因在鱼刚长鳞片的皮肤上表达较高,说明该基因可能参与鳞被发育;而同时在尾鳍、背鳍和腹鳍中也有特异的高表达信号,说明FGF1a可能也参与鳍条的发育过程。Fibroblast growth factor 1a （FGF1a）, an important class of growth factor, plays an important role in the process of cell development. The full length CDS ofFGF1a gene was obtained using the specific primers designed refer to the common carp FGF1a EST sequence in our genome dataset. The FGF1a protein of common carp comprised of 146 amino acids, along with a receptor binding site. After sequence alignments among several species, the FGF1a from common carp is found to share the highest similarity. However, the similarities among the common carp and the human, mouse, xenopus and chicken are lower. The FGF1a in common carp was found to express specifically in developing scale tissues by RNA in situ hybridization, while it was also observed in the tissues of tail and the dorsal or ventral fins. All of these results provide us with the vital roles of FGF1a gene in earlier development of scales and fins.

关 键 词：FGF1a镜鲤 鳞被 原杂技术 

分 类 号：S965.116[农业科学—水产养殖]