用ITS序列识别牧草种质资源  

Identification of Forage Germplasm Resources in ITS Sequence

作　　者：姚娜[1] 谭嘉力[2] 赖志强[1] 韦锦益[1] 徐海雄[1] 

机构地区：[1]广西壮族自治区畜牧研究所,南宁530001 [2]广东产品质量监督检验研究院,国家食品质量监督检验中心(广东),顺德528300

出　　处：《基因组学与应用生物学》2014年第1期141-144,共4页Genomics and Applied Biology

基　　金：广西水产畜牧兽医局科技计划项目(桂渔牧科1304511)资助

摘　　要：DNA条形码技术能够快速、准确地识别物种,对于开展基础性的分类学研究和应用性的生物多样性研究极为重要。本文以广西畜牧研究所牧草种质资源圃的8个物种共22个品种为材料,进行植物条形码ITS测序。PCR结果显示ITS的扩增成功率达到100%,同源性的分析表明各物种中的ITS序列变异显著,共找到99个可以用来区分物种的变异位点,系统进化树的结果表明利用ITS序列进行物种水平鉴定成功率达100%,能够作为DNA条形码识别植物物种。It is crucial for the studies of taxonomy and biodiversity by using DNA barcode technology to fast and accurately make species identification. In this study, the 22 varieties from 8 plant species in Guangxi Institute of Animal Science were sampled and sequenced. Plant samples were processed with a classical DNA barcoding approach by amplifying and sequencing for ITS, and obtained 99 SNP to species identification. The SNP and neighbour-joining tree were employed to test the reliability ofbarcode at the species level. The results showed that ITS had significant divergence and high species identification reliability （100%）, and it was recommended as a test fragment ofbarcoding to species identification.

关 键 词：DNA条形码 核糖体内转录间隔区(ITS) 物种鉴别 

分 类 号：S54[农业科学—作物学]