Phylogenetic analysis and in vitro culture of mosses fromthe Antarctic Fildes Peninsula  

作　　者：LIU Shenghao ZHANG Zhaohui WANG Nengfei CONG Bailin ZHANG Pengying LIN Xuezheng HUANG Xiaohang 

机构地区：[1]Marine Ecology Research Center,The First Institute of Oceanography, State Oceanic Administration [2]College of Life Science, Shandong University

出　　处：《Advances in Polar Science》2014年第2期97-104,共8页极地科学进展（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Grant nos.40906103 and 41206176);the Chinese Polar Environment Comprehensive Investigation & Assessment Programs(Grant nos.CHINARE2012-02-01 and CHINARE2012-01-06);the Ocean Public Welfare Scientific Research Project of China(Grant no.201005031)

摘　　要：Molecular genetic techniques have proven very useful for initial analysis of the extent of genetic variation and dispersal in several Antarctic moss species. In the present study, the small subunit ribosomal RNA （SSU rDNA） and internal transcribed spacers of the nuclear ribosomal DNA （ITS rDNA） were sequenced in nine individuals of different mosses from the Fildes Peninsula of Antarctica. Sequence alignment showed that the extreme environment tended to increase the genetic diversity of Antarctic mosses. In addition, in our phylogenetic analysis, one previously unidentified Antarctic moss species was characterized by comparison with SSU and ITS rDNA sequences of known moss species. Moreover, the optimal culture medium and conditions for surface explant sterilization and protonemata induction in tissue culture of Pohlia nutans were investigated. The successful establishment of a tissue culture protocol together with the phylogenetic analysis of Antarctic mosses will provide technological support to establish an effective resource regeneration method for discovering new functional genes and gaining novel insights into the mechanisms of stress acclimation.Molecular genetic techniques have proven very useful for initial analysis of the extent of genetic variation and dispersal in several Antarctic moss species. In the present study, the small subunit ribosomal RNA （SSU rDNA） and internal transcribed spacers of the nuclear ribosomal DNA （ITS rDNA） were sequenced in nine individuals of different mosses from the Fildes Peninsula of Antarctica. Sequence alignment showed that the extreme environment tended to increase the genetic diversity of Antarctic mosses. In addition, in our phylogenetic analysis, one previously unidentified Antarctic moss species was characterized by comparison with SSU and ITS rDNA sequences of known moss species. Moreover, the optimal culture medium and conditions for surface explant sterilization and protonemata induction in tissue culture of Pohlia nutans were investigated. The successful establishment of a tissue culture protocol together with the phylogenetic analysis of Antarctic mosses will provide technological support to establish an effective resource regeneration method for discovering new functional genes and gaining novel insights into the mechanisms of stress acclimation.

关 键 词：phylogenetic tree tissue culture protonemata formation Pohlia nutans 

分 类 号：Q949.35[生物学—植物学] X511[环境科学与工程—环境工程]