Modification and Evaluation of Brucella Broth Based Campylobacter jejuni Transport Medium  

作　　者：BAI Yao CUI Sheng Hui XU Xiao LI Feng Qin 

机构地区：[1]Key Laboratory of Food Safety Risk Assessment, National Health and Family Planning Commission, China NationalCenter for Food Safety Risk Assessment [2]National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention [3]National Institutes for Food and Drug Control

出　　处：《Biomedical and Environmental Sciences》2014年第6期466-470,共5页生物医学与环境科学（英文版）

基　　金：supported by grant(2012AA101603)from the Ministry of Science Technology;grant(2011X7)from the National Institute of Food and Drug Control;grant(81102131)from the National Natural Science Foundation of China

摘　　要：Reliable transport of Campylobocter jejuni isolates is critical to microbial epidemiology research, especially in developing countries without a good temperature control mailing system. Various factors, including oxygen, temperature, transport medium composition, could affect the survival of C jejunL In this study, the protective effects of different ingredients in C. jejuni transport media at 4 ℃ and 25 ℃ and under aerobic condition were quantitatively evaluated respectively. The results showed that enriched medium, supplementation with 5% blood and being kept at 4 ℃ could improve the viability of different C. jejuni strains during transport. In addition, supplementation with 25 mmol/L L-fucose in Wang＇s transport medium could significantly improve the survival of C.Reliable transport of Campylobocter jejuni isolates is critical to microbial epidemiology research, especially in developing countries without a good temperature control mailing system. Various factors, including oxygen, temperature, transport medium composition, could affect the survival of C jejunL In this study, the protective effects of different ingredients in C. jejuni transport media at 4 ℃ and 25 ℃ and under aerobic condition were quantitatively evaluated respectively. The results showed that enriched medium, supplementation with 5% blood and being kept at 4 ℃ could improve the viability of different C. jejuni strains during transport. In addition, supplementation with 25 mmol/L L-fucose in Wang＇s transport medium could significantly improve the survival of C.

关 键 词：Modification and Evaluation of Brucella Broth Based Campylobacter jejuni Transport Medium 

分 类 号：R446.5[医药卫生—诊断学]