基于代谢组学技术对骨蚀灵胶囊干预激素诱发股骨头坏死的机制探讨  

Metabonomic study on the mechanism of steroid-induced avasular necrosis of femoral head with Gushiling Capsule

作　　者：于雪峰[1] 彭维霞[1] 陈凯云[1] 吕岫华[2] 樊祥伟[1] 徐江霞[1] 刘伟[2] 艾萍[2] 李小萌[2] 邢丽颖[2] 黄嘉瑾 

机构地区：[1]南昌大学第四附属医院,南昌330003 [2]北京工业大学生命科学与生物下程学院,北京100124

出　　处：《中华中医药杂志》2014年第6期1805-1808,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81241141)~~

摘　　要：目的:探讨激素诱发股骨头坏死脂质代谢变化规律,寻求中药干预作用"靶点"及作用机制。方法:通过激素诱发股骨头坏死实验兔模型,基于超高效液相色谱(UPLC)技术,观察与体内脂质代谢密切相关的游离脂肪酸(FFA)在激素作用下的变化规律;生化分析对血清脂质和脂蛋白的影响;观察股骨头骨组织形态学的相应改变。结果:在3周和5周的时间点上,实验动物血浆中FFA呈先高后低现象,3周时与正常对照组比较,均显著升高(P<0.01);第5周时中药组、西药组的FFA仍高于模型组(P<0.05);血浆中的胆固醇(TC)、三酰甘油(TG)呈相应变化;模型组骨组织病理有骨坏死表现。结论:FFA代谢异常是激素诱发股骨头坏死的重要病理基础;中药通过调节脂质代谢可以早期预防激素性股骨头坏死。Objective： To investigate the lipid metabolism change rule in the progresssion of steroid-induced avasular necrosis of femoral head, and look for the target of Chinese medicine intervention, observe the effect of early intervention. Methods： After setting up rabbit model of steroid-induced avasular necrosis of femoral head, we observe the change rule of free fatty acids with hormone that closely related lipid metabolism; analyze the influence such as serum lipid, proteolipid protein by laboratory tests; and observe appropriate change under filters and electron microscope of local femoral head. Results： The free fatty acids of experimental animals increased before decreasing in the time of 3 weeks and 5 weeks, compared with normal group, it＇s increased significantly in 3 weeks （P〈0.01）, while the free fatty acids of Chinese medicines group and western group still higher than model group （P〈0.05） in 5 weeks. Cholesterol and triacylglycerol was related positively to the changes of free fatty acids, and pathology examination found osteonecrosis femoral head in model group. Conclusion： Abnormal metabolism of free fatty acid is an important pathological basis of femoral head necrosis. Traditional Chinese medicine can prevent early of femoral head necrosis by regulate lipid metabolism.

关 键 词：股骨头坏死 激素 代谢组学 游离脂肪酸 中药 

分 类 号：R285.5[医药卫生—中药学]