黄芪和三七4种有效成分配伍对小鼠脑缺血再灌注早期抗氧化应激物质活性的影响  

Influence of compatibility of four active components of astragalus and notoginseng on the active substances of anti-oxidative stress in the early stage of cerebral ischemiareperfusion of mice

作　　者：邱咏园[1] 唐映红[1] 王蓓[1] 曾嵘[1] 邓常清[1] 黄小平[1] 

机构地区：[1]湖南中医药大学,长沙410208

出　　处：《中华中医药杂志》2014年第6期1940-1943,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81102557);教育部2010年度高等学校博士学科点专项科研基金项目(No.20104323110001);湖南省高校创新平台开放基金项目(No.11K050);湖南省中医药管理局重点项目(No.201301);湖南省教育厅一般项目(No.11C0963);湖南省科技厅一般项目(No.2014SK3001);湖南省高校科技创新团队支持计划~~

摘　　要：目的:观察黄芪和三七的4种有效成分黄芪甲苷、人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1配伍对小鼠脑缺血再灌注早期氧化应激物质活性的影响,并探讨其机制。方法:C57BL/6小鼠随机分组,灌胃给药,连续3d,末次给药1h后,制作脑缺血再灌注模型。取脑组织匀浆,测定脑组织还原型谷胱甘肽(GSH)、总超氧化物歧化酶(T-SOD)、总抗氧化能力(T-AOC)3种抗氧化物质活性的含量。结果:脑缺血再灌注后,脑组织T-AOC能力,T-SOD活性和GSH含量显著降低。4种有效成分配伍、黄芪甲苷+人参皂苷Rg1、黄芪甲苷+人参皂苷Rb1能显著提高脑组织T-SOD活性;黄芪甲苷、4种有效成分配伍、黄芪甲苷+人参皂苷Rg1、黄芪甲苷+人参皂苷Rb1、黄芪甲苷+三七皂苷R1组能显著增加脑组织GSH含量;人参皂苷Rg1、三七皂苷R1、4种有效成分配伍、黄芪甲苷+人参皂苷Rg1、黄芪甲苷+人参皂苷Rb1、黄芪甲苷+三七皂苷R1组显著升高脑组织T-AOC能力。且黄芪甲苷+人参皂苷Rg1、黄芪甲苷+人参皂苷Rb1、黄芪甲苷+三七皂苷R1组升高GSH含量的效应分别大于人参皂苷Rg1,人参皂苷Rb1、三七皂苷R1单用组,升高T-AOC的效应高于黄芪甲苷组和人参皂苷Rb1单用组。4种有效成分配伍组与人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1组比较,脑组织T-SOD活性增加更显著,与黄芪甲苷、人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1组和黄芪甲苷+三七皂苷R1组比较,脑组织GSH含量增加显著,与黄芪甲苷、人参皂苷Rb1组比较,脑组织T-AOC能力增加显著。结论:黄芪甲苷与人参皂苷Rg1,人参皂苷Rb1,三七皂苷R1合用后,能同时提高T-SOD活性、GSH含量和T-AOC能力,对脑缺血再灌注后氧自由基的清除和抗氧化应激损伤具有增强的作用。且对T-SOD的作用主要来自4种有效成分的合用,对GSH的作用主要来自黄芪甲苷,对T-AOC的作用主要来自人参皂苷Rg1和三七皂苷R1。Objective： To study the influence and mechanism of four active components combination of astragalus and ginsenoside, such as astragaloside, ginsenoside Rgl, Rbl, and notoginseng R1 on the active substance to against oxidative stress in the early stage of cerebral ischemia-repeffusion with mice. Methods： C57BL/6 mice were randomly assigned, and proceeded intragastric administration for 3 days continuously. Cerebral ischemia-reperfusion model was established after 1 hour at the late administration. Brain tissue homogenate was made to detect the contents of glutathione （GSH）, total antioxidant capacity （T-AOC） and the activity of total superoxide dismutase （T-SOD）. Results： After cerebral ischemia-reperfusion, T-AOC ability, T-SOD activity and GSH content in brain tissue were decreased significantly. T-SOD activity was increased significantly by compatibility of four active component, compatibility of astragaloside 1V and ginsenoside Rg i and compatibility of astragaloside IV and ginsenoside Rbl. GSH contents were increased significantly by in astragaloside IV, compatibility of four active component, compatibility of astragaloside IV and ginsenoside Rgl, compatibility of astragaloside IV and ginsenoside Rbl and compatibility of astragaloside IV and notoginsenoside RI. T-AOC capacity was increased significantly by ginsenoside Rgl, notoginsenoside R 1, compatibility of four active component, compatibility of astragaloside IV and ginsenoside Rgl, compatibility of astragaloside IV and ginsenoside Rbl, compatibility of astragaloside IV and notoginsenoside RI. Compare with compatibility of astragaloside IV and ginsenoside Rgl, compatibility of astragaloside 1V and ginsenoside Rbl and compatibility of astragaloside IV and notoginsenoside R1 alone, the increased effect on GSH of ginsenoside Rgl, Rbl and notoginsenoside R1 alone was less, and the increased effect on T-AOC of astragaloside IV and ginsenoside Rbl alone was less. Compare with compatibility of four active component, the active of T-SOD was less

关 键 词：抗氧化应激活性物质 黄芪 三七 有效成分 脑缺血再灌注损伤 

分 类 号：R285.5[医药卫生—中药学]