乙型肝炎肝硬化湿热证与肿瘤坏死因子α基因多态性相关性研究  

Research on the association between hepatitis B liver cirrhosis of dampness-heat syndrome and tumor necrosis factor-α gene polymorphism

作　　者：石志平[1] 吴同玉[1] 刘瑶[1] 沈建英[1] 廖凌虹[1] 李东良[2] 张凌媛[1] 

机构地区：[1]福建中医药大学,福州350122 [2]南京军区福州总医院,福州350025

出　　处：《中华中医药杂志》2014年第6期2004-2006,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年基金项目(No.81102559)~~

摘　　要：目的:研究福建人群中乙型肝炎肝硬化湿热证与肝硬化易感基因肿瘤坏死因子α(TNF-α)基因多态性的关系。方法:选用福州地区80例乙型肝炎肝硬化病例为研究对象,收集资料,根据证素积分法将其分为湿热证组(湿证素积分和热证素积分均≥100)、非湿热证。应用聚合酶链反应-限制性片段长度多态性(PCRRFLP)方法分析TNF-α基因多态性。采用卡方检验分析基因分型与乙型肝炎肝硬化湿热证的关系。结果:TNFα-308基因多态性中,湿热证组的GG基因型、GA基因型与非湿热组比较,差异有统计学意义(P<0.05);湿热证组G等位基因、A等位基因与非湿热组比较,差异有统计学意义(P<0.05)。TNFα-238在湿热证组、非湿热证证组之间差异无统计学意义。结论:TNF-α-308G/A的基因多态性可能与乙肝肝硬化的遗传易感性有关,TNF-α-308A可能是乙肝肝硬化患者湿热证的潜在遗传学因素。Objective： To study the relationship between hepatitis B liver cirrhosis of dampness-heat syndrome and the liver cirrhosis susceptibility genes tumor necrosis factor-α （TNF-α） gene polymorphism in Fujian people. Methods： 80 cases of hepatitis B liver cirrhosis patients that come from Fuzhou region were selected, according to the syndrome element integration methods, the patients were divided into dampness-heat syndrome group （dampness syndrome factor scores and heat syndrome factor were 100 or higher） and non dampness-heat syndrome group after collected the data. Application of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method to analyze the TNF-α gene polymorphism. Chisquare test was used to analyze the relationship between the gene typing and the hepatitis B liver cirrhosis of dampness-heat syndrome. Result： There were significant differences of GG genotype and GA genotype of the tumor necrosis factor-or-308 gene polymorphism between the dampness-heat syndrome group and the non dampness-heat syndrome group （P〈0.05）, there were significant differences of G allele and A allele between the dampness-heat syndrome group and the non dampness-heat syndrome group （P〈 0.05）. The tumor necrosis factor-or-308 gene polymorphism was no statistically significant between the dampness-heat syndrome group and non dampness-heat syndrome group. Conclusion： The tumor necrosis factor-or-308 G/A gene polymorphism may be associated with genetic susceptibility to hepatitis B liver cirrhosis, and may be a potential genetic factor to the hepatitis B liver cirrhosis of dampness-heat syndrome.

关 键 词：乙型肝炎肝硬化 湿热证 肿瘤坏死因子Α 基因多态性 

分 类 号：R259[医药卫生—中西医结合]