三七总皂苷拮抗Nogo-A抑制神经细胞生长作用实验研究  

Effect of Xuesaitong on Nogo-A Induced Injury on SH-SY5Y Cell

作　　者：郜佳佳 张晓萌[1] 刘丽星[1] 朱陵群[1] 陈家旭[2] 赵歆[2] 

机构地区：[1]北京中医药大学东直门医院中医内科学教育部重点实验室和北京市重点实验室,北京100700 [2]北京中医药大学基础医学院中医诊断系,北京100029

出　　处：《辽宁中医药大学学报》2014年第7期34-36,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30772803;81173235);北京中医药大学创新团队资助项目(2011CXTD-07)

摘　　要：目的:观察三七总皂苷对Nogo-A抑制神经细胞生长的拮抗作用。方法:选用人神经细胞系SHSY5Y细胞,采用Nogo-A(20×10-3μmol·L-1)对细胞产生抑制作用的方法,将细胞分为正常对照组,Nogo-A组,三七总皂苷大、小剂量组,Nogo-A+三七总皂苷大、小剂量组,维甲酸(retinoic acid,RA)组。用CCK-8法于加药后不同时间点检测细胞活力;并在倒置相差显微镜下观察加药后72 h的SH-SY5Y细胞的形态及轴突生长情况。结果:Nogo-A组对细胞活性及轴突生长产生明显抑制,胞体皱缩、变圆,突起减少,部分细胞漂浮于培养基中,而Nogo-A+三七总皂苷大、小剂量组细胞活性明显升高,细胞轴突伸长,仅有少量细胞胞体皱缩,细胞轮廓清晰,贴壁良好。且Nogo-A+三七总皂苷大剂量组细胞活性及轴突生长阳性细胞百分率高于Nogo-A+三七总皂苷小剂量组(P<0.05)。结论:三七总皂苷可以起到拮抗Nogo-A对神经细胞生长的抑制作用,促进轴突的生长,并存在一定的量效关系。Objective： To observe the effect of Panax notoginseng saponins injection on the Nogo-A induced injury of the SH-SYSY ceils. Method ： Using Nogo-A to induce the inhibitory effect on the human neuroblastoma SH-SYSY cells, the protective effect of different doses of Panax notoginseng saponins was observed. The samples were divided into normal control group, model group, the Panax notoginseng saponins high-dose group and low-dose groups, Panax notoginseng saponins high-dose and low-dose plus Nogo-A groups, and RA group. We used CCK-8 method 1o detecl cell proliferation and observed cell axon growth situation under inverted phase contrast microscope. Results： Nogo-A model group can cause obvious damage, cell body shriveled; became round and protuberant; some cells floated in the medium; cell activity and cell axon growth percentage were lower than those of the normal control group. Nogo-A plus Panax notoginseng saponins group＇s cell activity was slightly higher and axon growth was slightly elongate; only a few cells shriveled, the contour was clear, sticked well. There was a significant difference between the Nogo-A plus Panax notoginseng saponins high-dose group and low-dose group （ P〈0.05 ） .Cotmlusion ： Panax notoginseng saponins can protect ceils from the Nogo-A inhibition, and promote the growth of axons. There is a certain amount of activity relationship.

关 键 词：SH-SY5Y细胞 NOGO-A 三七总皂苷 轴突生长 

分 类 号：R28[医药卫生—中药学]