半夏厚朴汤抗抑郁作用——改善脑内氧化应激水平  

Antidepressant-like Effects of Banxia Houpu Decoction by Improving the State of Oxidative Stress in the Brain

作　　者：马占强[1] 李瑞鹏[1] 李月碧 傅强[1] 瞿融[2] 马世平[1] 

机构地区：[1]中国药科大学中药学院中药药理教研室,南京211198 [2]南京中医药大学方剂教研室,南京210046

出　　处：《药学与临床研究》2014年第3期205-208,共4页Pharmaceutical and Clinical Research

基　　金：江苏省自然科学基金面上项目资助(BK2011630);中央高校基本科研业务费专项资金资助(JKQ2011036);江苏高校优势学科建设工程项目(PAPD);江苏省普通高校研究生科研创新计划(CXZZ13_03)

摘　　要：目的:通过观察半夏厚朴汤对慢性应激抑郁模型大鼠行为学和氧化应激的影响,研究半夏厚朴汤抗抑郁的机制。方法:采用强迫游泳实验、开野实验、糖水偏好实验,研究半夏厚朴汤对慢性应激抑郁模型大鼠的抗抑郁作用,并通过UPLC-T-QMS系统检测其大鼠脑内单胺类神经递质的含量,部分动物取其海马制备海马匀浆,Western blotting检测超氧化物歧化酶(SOD)、丙二醛(MDA)。结果:与空白组相比,模型组大鼠糖水消耗量显著下降,强迫游泳不动时间显著延长,交叉次数、直立次数显著减少;海马内去甲肾上腺素、5-羟色胺含量显著降低。与模型组相比,半夏厚朴汤组糖水消耗量显著升高,强迫游泳不动时间显著缩短。半夏厚朴汤组显著增加大鼠脑内去甲肾上腺素和5-羟色胺含量。Western blotting检测结果显示,与模型组比较,半夏厚朴汤组显著升高SOD水平,显著降低MDA水平。结论:半夏厚朴汤能明显改善慢性应激所致的大鼠抑郁行为,其机制可能与上调海马内去甲肾上腺素、5-羟色胺水平,增强机体抗氧化应激能力有关。Objective： To study the anti-depression mechanism of Banxia Houpu Decoction. Methods：Rat models were established by chronic unpredictable mild stress（CUMS）, sucrose consumption, open-field and forced swimming tests were used to observe the behavior of the model rats. UPLC-T-QMS was used to analyze the levels of monoamine neurotransmitters. Western blotting detected the levels of superoxide dismutase（SOD） and malondialdehyde（MDA） in brain. Results： Compared with the normal group, the frequency of crossing and standing and the consumption of sucrose water in the depressive disorder rats decreased significantly, the duration of immobility in forced swimming test increased significantly, the contents of 5-HT and NA reduced. Compared with the model group, the frequency of crossing and standing and the consumption of sucrose water in the Banxia Houpu Decoction group rats increased significantly, the duration of immobility in forced swimming test decreased significantly. Western blotting analysis showed that,compared with the model group, Banxia Houpu Decoction could significantly elevate the levels of SOD, and significantly reduce the levels of MDA in hippocampus. Conclusion： Banxia Houpu Decoction improved the depression syndromes of rats induced by CUMS, of which the mechanism involved the upregulation of 5-HT and NE levels and enhancement of anti-oxidative stress ability.

关 键 词：半夏厚朴汤 抗抑郁 神经递质 氧化应激 

分 类 号：R965.1[医药卫生—药理学]