双波长HPLC法同时测定妇科Ⅳ颗粒剂中芍药苷和虎杖苷的含量  

Simultaneous Determination of Paeoniflorin and Polydatin in FukeⅣ Granules by Dual-wavelength HPLC

作　　者：钱俊[1] 林夏[1] 王星星[1] 刘莉莉[1] 吴云[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室,连云港222001

出　　处：《药学与临床研究》2014年第3期219-221,共3页Pharmaceutical and Clinical Research

摘　　要：目的：建立妇科Ⅳ颗粒剂中芍药苷和虎杖苷含量的HPLC测定方法。方法：采用Phenomenex Luna C18（4.6 mm×250 mm,5μm）色谱柱;流动相为乙腈-0.1%磷酸（17∶83）;流速为1.0 mL·min-1;柱温为30℃;检测波长为230 nm、306 nm。结果：芍药苷在0.524-8.384μg、虎杖苷在0.344-5.502μg范围内,进样量与峰面积具有良好线性关系（r=0.9997,r=0.9999）,平均回收率分别为99.6%（RSD=0.92%）、100.3%（RSD=1.32%）。结论：研究建立的含量测定方法可靠、准确、重现性好,可用于该制剂的质量控制。Objective： An HPLC-UV method was developed for the determination of paeoniflorin and polydatin in Fuke Ⅳ granules. Methods： The optimal conditions of separation and detection were achieved on a Phenomenex Luna C18（4.6 mm×250 mm, 5 μm） analytical column with an mobile phase consisting of acetonitrile-0.1% phosphoric acid（17∶83） at the flow-rate of 1 mL·min-1, The column temperature was 35 ℃and the UV detection wavelength was 230 nm and 306 nm respectively. Results： The calibration curves for paeoniflorin and polydatin were linear over the range of 0.524 μg -8.384 μg and 0.344 μg -5.502 μg respectively. The recoveries were 99.6% and 100.3% with RSD 0.92% and 1.32%（n =6）, respectively.Conclusion： The method is simple and reliable with good reproducibility and can be used to control the quality of the granules.

关 键 词：高效液相色谱法 芍药苷 虎杖苷 

分 类 号：R927.2[医药卫生—药学]