模拟微重力效应对骨细胞Wnt/β-catenin通路的影响  

Effects of Simulated Microgravity on Wnt /β-catenin Pathway in Osteocytes

作　　者：梁萌[1] 杨肖[1] 樊瑜波[1] 孙联文[1] 

机构地区：[1]北京航空航天大学生物与医学工程学院,生物力学与力生物学教育部重点实验室,北京100191

出　　处：《航天医学与医学工程》2014年第3期164-167,共4页Space Medicine & Medical Engineering

基　　金：国家重点基础研究发展计划(973)资助项目(2011CB710901)

摘　　要：目的探究模拟微重力效应对骨细胞Wnt/β-catenin信号通路活性的影响,以阐明微重力导致骨质丢失的发生机制。方法将骨细胞MLO-Y4分为两组:模拟微重力组和静置对照组。细胞旋转培养以模拟微重力效应,利用RT-PCR和Western blot法检测细胞Wnt/β-catenin信号通路相关组分的基因和蛋白表达水平。结果 RT-PCR结果显示,模拟微重力组的Cyclin D1和Lef-1 mRNA水平显著低于对照组(P<0.05),CX43和抑制剂SOST的mRNA水平显著高于对照组(P<0.05),而两组的Wnt3a、Lrp5和β-catenin的mRNA水平无显著性差异。Western blot结果显示,模拟微重力组的β-catenin和Cyclin D1的蛋白表达水平显著低于对照组(P<0.05),Lef-1和抑制剂SOST蛋白质的表达水平显著高于对照组(P<0.05),而两组Wnt3a、Lrp5/6和CX43的蛋白表达水平无显著性差异。结论模拟微重力效应下骨细胞中Wnt/β-catenin信号通路的活性有所下降。Objective To explore the effects of simulated microgravity on the activity of Wnt/β-catenin signaling pathway in osteocytes for clarifying the occurance mechanism of bone lose induced by mierogravity. Methods MLO-Y4 osteocytes were divided into two groups： simulated microgravity group and control group. Cells were cultured with rotation to simulate microgravity. RT-PCR and Western blot were used to examine components of gene and protein expression level related to Wnt/β-catenin signaling pathway. Results The results of simulated microgravity group to be compared with control group： the mRNA＇ s expression of Cyclin D1 and Lef-1 was significantly decreased （ P 〈 0.05）, but of CX43 and SOST was significantly increased （ P 〈 0.05 ）, as well as the expression of WntSa, Lrp5 and β-eatenin had no significant difference; Moreover, the protein＇ s expression of β-catenin, Cyelin D1 and CX43 was significantly decreased （ P 〈 0. 05 ） , but of Lef-1 and Selerostin was significantly increased （ P 〈 0.05 ）, as well as the expression of Wnt3 a, Lrp5/6 and CX43 had no significant difference. Conclusion The activity of Wnt/β-catenin signaling pathway declines in osteocytes under simulated microgravity.

关 键 词：模拟微重力 骨细胞 骨丢失 Wntβ-catenin通路 

分 类 号：R854[医药卫生—航空、航天与航海医学]