被动运动对抗尾吊大鼠软骨退化的效果  

Effects of Passive Exercise on Counteraction of Articular Cartilage Degeneration in Tail-suspended Rats.

作　　者：栾会芹[1] 黄云飞[1] 樊瑜波[1] 孙联文[1] 

机构地区：[1]北京航空航天大学生物与医学工程学院,生物力学与力生物学教育部重点实验室,北京100191

出　　处：《航天医学与医学工程》2014年第3期172-175,共4页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助项目(31170897)

摘　　要：目的探寻后肢去负荷过程中,能够阻止软骨退化的对抗措施。方法 SD大鼠分3组(每组8只):对照组(Con)、尾吊组(TS)、尾吊+被动运动组(TSP)。尾吊21天期间,TSP组接受由训练装置提供的被动运动训练。利用μCT与组织切片检测大鼠股骨远端软骨的变化。结果μCT结果表明,TS与TSP组大鼠股骨远端的关节软骨厚度与Con比明显下降;TS的软骨体积较Con与TSP显著下降,而Con与TSP间没有显著差异;TS与TSP的软骨的衰减系数较Con明显升高。组织切片表明,TS与TSP的软骨厚度较Con明显下降,番红O—固绿染色显示TS与TSP的基质染色程度较Con下降,软骨细胞出现减少的现象。此外,μCT所测厚度与组织切片所测厚度高度相关。结论大鼠尾吊会导致关节软骨退化,然而被动运动不能有效的阻止尾吊引起的软骨退化。Objective To explore the effects of passive exercise on counteraction of articular cartilage degeneration in tail-suspended rats. Methods Twenty four female Sprague Dawley rats were randomly divided into three groups（n =8, each）： control（Con）, tail-suspension（TS） , and tail-suspension plus passive motion （TSP）. In TS and TSP groups, rat hindlimbs were unloaded for 21 days by tail suspension. During these 21 days, rats of TSP groups carried out the passive motion exercises with a training apparatus. Following, the cartilage thickness, volume and attenuation of the distal femur were evaluated with μCT. Histological analysis was used to assess the surface integrity of cartilage, cartilage thickness and chondrocytes. Results μCT showed that cartilage thickness of the distal femur decreased significantly in TS and TSP groups when compared with Con group. Cartilage volume in the TS group decreased significantly compared with Con and TSP groups. Histology images showed that the degree of matrix staining was decreased in TS and TSP groups. Additionally, the cartilage thickness measurement from μCT and histology images showed good correlation. Conclusion The results indicate that tail suspension can induce articular cartilage degeneration. This passive exercise is ineffective in preventing such degeneration in tail-suspended rats.

关 键 词：模拟失重 被动运动 关节软骨 软骨退化 μCT 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]