骨代谢生化指标测定在老年人骨质疏松症中的应用  

Application of bone metabolic biochemical markers in the elderly patients with osteoporosis

作　　者：吴显劲[1,2] 梁栋伟[3] 李秋丽[4] 徐军发[5] 

机构地区：[1]暨南大学附属第一医院,广东广州510630 [2]广东医学院附属医院检验科,广东湛江524001 [3]佛山市南海区第二人民医院检验科,广东佛山258251 [4]广州金域医学检验中心,广东广州510330 [5]广东医学院检验医学研究所,广东东莞523808

出　　处：《中国卫生检验杂志》2014年第11期1604-1605,1608,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨骨代谢生化指标测定在老年人骨质疏松症中的应用。方法采用酶联免疫吸附法(ELISA)测定抗酒石酸酸性磷酸酶-5b(TRACP-5b)、骨特异性碱性磷酸酶(BALP)和骨钙素N端中分子片段(N-MID);用化学发光免疫法定量测定25-羟维生素D3[25-(OH)-VD3]。结果骨质疏松女性组除TRACP和25-(OH)-VD3外,N-MID与对照女性组有明显的降低(P<0.05),而BALP与对照女性组有明显的升高(P<0.05)。骨质疏松男性组除25-(OH)-VD3外,N-MID与对照男性组的有明显的降低(P<0.05),而TRACP和BALP与对照男性组的均有明显的升高(P<0.05)。结论测定血清中的TRACP-5b、BALP、N-MID可在一定范围内了解体内骨代谢情况,并可作为骨质疏松症的检测指标。Objective To evaluate the role of bone metabolic biochemical markers in the elderly patients with osteoporosis.Methods Serum tartrate-resistant acid phosphatase-5b（TRACP-5b）,bone alkaline phosphatase（B-ALP）,N-MID osteocalcin were tested by ELISA and the 25-（OH）-VD3 was tested by chemiluminescence immunoassay（CLIA）.Results Except for TRACP-5b and 25-（OH）-VD3,N-MID in women patients with osteoporosis was obviously lower compared with the women control group（P〈0.05）,but the BALP was higher（P〈0.05）.While except for 25-（OH）-VD3,the level of N-MID in men patients was lower than that in men control group（P〈0.05）,but the levels of TRACP-5b and BALP were higher（P〈0.05）.Conclusion It is found that determination of serum TRACP,BALP,N-MID may contribute to understand the bone metabolism condition of people,and it can be regard as the detection index of osteoporosis.

关 键 词：骨质疏松症 抗酒石酸酸性磷酸酶-5b 骨碱性磷酸酶 骨钙素N端中分子片段 25-羟维生素D3 

分 类 号：R681[医药卫生—骨科学]