适宜不同种类细胞的温敏性共聚物膜的筛选  

Selection of Temperature-Responsive Copolymer Film for Different Cells

作　　者：杨磊[1] 王战勇[1] 范晓光 

机构地区：[1]辽宁石油化工大学环境与生物工程学院,辽宁抚顺113001 [2]中国石油抚顺石化分公司,辽宁抚顺113008

出　　处：《辽宁石油化工大学学报》2014年第3期14-18,32,共6页Journal of Liaoning Petrochemical University

基　　金：国家自然科学基金项目(31100099);辽宁石油化工大学科学基金项目(80040139)

摘　　要：以温敏性聚N-异丙基丙烯酰胺(poly(N-isopropylacrylamide),PNIPAAm)为主体的细胞培养基底通过温度的改变来调控细胞在其上的黏附和脱附,有效替代传统酶解法实现贴壁细胞的非酶解收获,同时,贴壁细胞对温敏性基底具有选择性。选取贴壁型HeLa细胞、HEK293细胞、骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMMSCs)和脂肪干细胞(adipose-derived stem cells,ADSCs)作为考察对象,采用具有不同表面润湿性、表面形貌和膜厚度等特征的系列PNIPAAm共聚物膜作为备选培养基底,考察查适合这四种细胞黏附和脱附的共聚物膜性能范围。实验结果表明,不同种类细胞对温敏性基底具有不同的选择性,同时为上述细胞分别选取了适宜的温敏性共聚物膜。Temperature-responsive culture substrates containing poly（N-isopropylacrylamide）（PNIPAAm）have been developed as an effective substitute for enzymatic treatment to recover adherent cells,which can control cell attachment and detachment by changing temperature.However,not all PNIPAAm-containing surfaces can be applicable to cell culture and recovery.That means adherent cells show accommodative selectivity for thermo-responsive substrates.In this study,different cells including HeLa cells,HEK293 cells,bone marrow mesenchymal stem cells（BMMSCs）and adipose-derived stem cells（ADSCs）were cultured on the temperature-responsive PNIPAAm copolymer films with different surface wettability,topography and film thickness.The experimental results demonstrate that various cells had their special thermo-responsive substrates,and the optimal copolymer films were selected for HeLa cells,HEK293 cells,BMMSCs and ADSCs respectively.

关 键 词：聚N-异丙基丙烯酰胺 骨髓间充质干细胞 脂肪干细胞 温敏性材料 非酶解收获 

分 类 号：TQ813.11[化学工程]