LC-MS/MS在冠心舒胶囊大鼠体内药动学中的应用  

Application of LC-MS/MS on pharmacokinetics of Guanxinshu Capsule in rats

作　　者：陈俊杰[1] 王丹辉 傅国强[1] 候莉[1] 何秀菊[1] 杨世林[1] 范玫玫[1] 

机构地区：[1]江西中医药大学,江西南昌330006 [2]解放军94医院,江西南昌330053

出　　处：《药物评价研究》2014年第3期227-230,共4页Drug Evaluation Research

基　　金：江西省青年科技基金(20114BAB205077)

摘　　要：目的建立测定丹参素在大鼠血浆中浓度的LC-MS/MS方法,并将该方法用于冠心舒胶囊在大鼠体内的药动学。方法色谱柱为C18柱(50 mm×2.1 mm,1.7μm),采用梯度洗脱的方式分离被测物质,利用液液萃取的方法处理血浆样品,以多反应监测(multiple reaction monitoring,MRM)扫描方式检测。结果丹参素在线性范围内线性关系良好,日内日间精密度(RSD)均小于15%,准确度(RE)在±15%以内,平均提取回收率在80%以上,基质效应在90%～110%。结论该方法适合于冠心舒胶囊在大鼠体内的药动学研究。Objective To establish an LC- MS/MS method for determing danshensu（DSS） in rat plasma, and to apply the method to the pharmacokinetics of Guanxinshu Capsule in rats. Methods The analytes were separated on a C18 column（50 mm × 2.1 mm, 1.7 μm） and a triple-quadrupole mass spectrometry equipped with electrospray ionization（ESI） source was applied for detection. The liquid-liquid extraction cartridge was employed to extract the analyte from rat plasma, and multiple reaction monitoring was used to detect the samples. Results The method was linear over the concentration ranges of 1—1 000 ng/mL for DSS. The intra- and inter-day relative standard deviation（RSD） were less than 15% and the relative errors were all within 15%, with the recovery of DSS（〉 80%） and matrix effect of 90%—110%. Conclusion The method is successfully applied to the pharmacokinetic study of Guanxinshu Capsule in rat.

关 键 词：液质联用 丹参素 大鼠血浆 液液萃取 药动学 

分 类 号：R965[医药卫生—药理学]