小檗碱与罗格列酮干预高脂饮食诱导大鼠非酒精性脂肪性肝炎的比较观察  

Comparison of the Efficacy between Berberine and Rosiglitazone in Non-alcoholic Steatohepatitis Rats Induced by High Fat Fiet

作　　者：张慧芹[1] 刘泽洲[1] 续畅[1] 李健[1] 牛建昭[1] 郝钰[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《现代生物医学进展》2014年第20期3806-3809,3839,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(30873268);高等学校学科创新引智计划资助项目(B07007)

摘　　要：目的:比较中药单体化合物小檗碱和噻唑烷二酮类药物罗格列酮对高脂饲料诱导大鼠非酒精性脂肪性肝炎(NASH)的干预作用,探讨小檗碱成为天然胰岛素增敏剂的可能性。方法:雄性SD大鼠40只,随机分为4组,采用连续饲喂高脂饲料的方法诱导大鼠NASH,以预防给药的方式灌胃给予小檗碱(100 mg/kg体重)和罗格列酮(20 mg/kg体重),持续8周后取材。采用生化分析的方法检测大鼠血清胆固醇(CHO)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、空腹血糖(FPG)及空腹胰岛素(FINS)并计算胰岛素抵抗指数(HOMA-IR)。采用常规石蜡切片HE染色、冰冻切片油红O染色评估NASH的病理程度,用常规免疫组织化学方法检测了肝组织中PPAR-γ的表达。结果:小檗碱和罗格列酮均能较好的干预高脂饲料诱导大鼠NASH的病理过程。此外,二者均能改善大鼠胰岛素抵抗状态、上调肝组织中PPAR-γ的水平。结论:小檗碱和罗格列酮均能较好的改善高脂饲料诱导的大鼠NASH病理过程,二者共同的药理机制是改善胰岛素抵抗状态和上调肝组织中PPAR-γ的表达。该实验结果提示:小檗碱有望开发为具有胰岛素增敏作用的天然药物。Objective： The study was to compare the efficacy of berberine and rosiglitazone in the treatment of non-alcoholic steatohepatitis （NASH） rats induced by high fat diet, so as to investigate the possibility of substituting rosiglitazone. Methods： 40 Male SD rats were randomly divided into 4 groups （10 rats per group）, i.e. the normal group （fed with normal diet）, the NASH model group （fed with high fat diet）, Rosiglitazone treatment group （20 mg/Kg body weight） and berberine treatment group （100 mg/Kg body weight）. Drugs were adopted in the preventive intervention method for 8 weeks. The hepatic histopathology method was adopted to evaluate the drug therapeutic effect. The serum levels of cholesterol （CHO）, triglyceride （TG）, high density lipoprotein （HDL）, low density lipoprotein （LDL）, fasting plasma glucose （FPG）, and fasting insulin （FINS） were examined with biochemical method. And then, HOMA-IR was calculated to show insulin resistance. And PPAR-3, expression on hepatic tissue was detected by immunohistochemistry method. Results： The results showed berberine and rosiglitazone could improve the degree of hepatic histopathology. Furthermore, both drugs can improve insulin resistance, and up-regulate the level of PPAR-γ on hepatic tissue. Conclusion： Berberine and rosiglitazone could alleviate the pathological process in high fat diet induced NASH model possibly through improving insulin resistance, up regulating PPAR-γ expression. The results suggest that berberine is expected to be developed into a natural drug with insulin sensitization effect.

关 键 词：小檗碱 罗格列酮 非酒精性脂肪性肝炎 大鼠 

分 类 号：R95-3[医药卫生—药学] R575.5