几种保存方法对中华哲水蚤和强壮箭虫体型的影响  

The influence of several preservation methods on the body size of Calanus sinicus and Sagitta crassa

作　　者：冯秋园[1,2] 孙晓霞[1] 任琳琳[1,2] 

机构地区：[1]中国科学院海洋研究所 山东胶州湾海洋生态系统国家野外科学观测研究站,山东青岛266071 [2]中国科学院大学,北京100049

出　　处：《海洋科学》2014年第4期20-26,共7页Marine Sciences

基　　金：国家重点基础研究发展计划项目(2014CB441504);中国科学院战略性先导科技专项(XDA05030401);国家海洋公益性行业科研专项(201005014)

摘　　要：选取5%的甲醛,–20℃冰冻和液氮为保存方法,以中国近海的2种优势浮游动物中华哲水蚤(Calanus sinicus)和强壮箭虫(Sagitta crassa)为研究对象,以体长、体宽和面积为体型参数,研究了上述几种保存方法在2个月的时间内对浮游动物体型的影响。研究结果显示,对于中华哲水蚤,甲醛保存2个月后,体长下降为原始体长的99.4%,体宽和面积都增加为107%;冰冻和液氮保存2个月后,体长分别增加为原始体长的101%和106.8%,体宽下降为90%和81.6%,面积下降为92.2%和88.8%。对于强壮箭虫,在甲醛、冰冻和液氮3种保存条件下,保存2个月后体长有所下降,分别为原始体长的92.8%,50.7%和48.4%。体宽和面积有所增加,体宽分别变为原始体宽的139.9%,159.4%和263.8%,面积分别变为原始面积的119%,97.8%和107%。综合分析发现,胶质类浮游动物在冰冻和液氮保存条件下,身体变形严重,因此用ZooScan测得的结果误差较大,相较而言,甲醛对胶质类浮游动物体型的影响较小;中华哲水蚤比强壮箭虫在各种保存条件下体型的变化要小;保存初期,浮游动物体型的变化幅度较大,后期趋于稳定;在3种保存条件下,中华哲水蚤和强壮箭虫体长的变化最稳定,其次是面积。对浮游动物的体型进行研究时,推荐使用甲醛保存条件下的体长或面积。In this paper, Calanus sinicus and Sagitta crassa two dominant species in coastal waters of China, were used to study the changes of body size （length, width and area） after 2 months preservation using three preservation methods including 5%formaldehyde,-20℃ freeze and liquid nitrogen. The results showed that for C. sinicus, the body length decreased 0.6%, and the body width increased 7%after preserved in formaldehyde for 2 months. The body length of C. sinicus preserved at -20℃ and in liquid nitrogen increased 1% and 6.8%, and the body width decreased 9%and 18.4%respectively. For S. crassa, the body length decreased, the body width and the body area increased a bit after preserved for 2 months. The body length decreased 7.2%, 49.3% and 51.6%, and the body width increased 39.9%, 54.4%and 163.8%, and the body area became 119%, 97.8%and 107%of the original ones, respectively after preserved in formaldehyde,-20℃ and liquid nitrogen for 2 months. The gelationous zooplankton became flexible, and deformed seriously when preserved at -20℃ and in liquid nitrogen, so that there were big errors when the body size was measured by zooscan. Comparing to freeze and Liquid nitrogen, formaldehyde led to smaller changes of zooplankton’s body size. The changes of body size of C.sinicus were smaller than that of S. crassa during the above three preservations. The body size changed dramatically at first, and then tended to stabi-lize. The body length changes of C. sinicus and S. crassa preserved in the above three ways were the smallest, fol-lowed by the area changes. Body length and area of zooplankton preserved in formaldehyde were recommended when the zooplankton body size were used after preservation.

关 键 词：胶州湾 浮游动物 保存方法 体型参数 

分 类 号：Q958.8[生物学—动物学]