柴芍安神解郁颗粒质量标准研究  

Study on quality standard for Chaishao Anshen Jieyu granules

作　　者：李丽娟[1] 彭丽诗[2] 李素梅[1] 罗文汇[1] 

机构地区：[1]广东省第二中医院(广东省中医药工程技术研究院),广东广州510095 [2]广州中医药大学,广东广州510405

出　　处：《湖南中医杂志》2014年第6期151-153,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：广东省中医药局资助项目(编号:20131110)

摘　　要：目的：建立柴芍安神解郁颗粒的质量控制标准。方法：采用薄层色谱法对制剂中的丹参、柴胡、石菖蒲3味药进行定性鉴别；采用高效液相色谱法对制剂中的丹酚酸B进行定量测定。结果：薄层色谱法可清晰鉴别丹参、柴胡和石菖蒲，且阴性对照无干扰；丹酚酸B在0．0518～0．5180μg范围内线性关系良好，相关系数r=0．9999，平均回收率为98．82％．RSD=1．27％。结论：本方法操作简单、专属性强、重现性好、数据准确，可用于疏肝安神的质量控制。Objective ：To establish the quality control standard for Chaishao Anshen Jieyu granules. Methods：Thin- layer chroma- tography（TLC） was used to identify Salvia mihiorrhiza, Bupleurum chinense, and Rhizoma Acori graminei in Chaishao Anshen Jieyu granules. High - performance liquid chromatography was used to determine the content of salvianolic acid B. Results ： Salvia miltiorrhiza, Bupleurum chinense, and Rhizoma Acori graminei were well identified by TLC, and the negative control had no interference. Salvianolic acid B showed a good linear relationship within O. 0518 -0. 5180 μg（ r = 0. 9999）. The average recovery rate was 98.82% , with a relative standard deviation of 1.27%. Conclusion：This method has the advantages of easy operation, high specificity, high repeatability, and high accuracy and can be used for the quality control of Chaishao Anshen Jieyu granules.

关 键 词：柴芍安神解郁颗粒 薄层色谱法 高效液相色谱法 丹酚酸B 质量标准 

分 类 号：R286.627[医药卫生—中药学]