促纤溶活性化合物FGFC1的分离纯化  

Separation and Purifi cation of Fibrinolytic Compound FGFC1

作　　者：刘永双[1] 那可[1] 赵文杰[1] 赵波[1] 魏晓东[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中国医药工业杂志》2014年第7期620-623,共4页Chinese Journal of Pharmaceuticals

基　　金：国家863计划项目(2011AA090702)

摘　　要：建立从发酵液中分离纯化高纯度促纤溶活性化合物FGFC1的方法。长孢葡萄穗霉Stachybotrys longispora FG216发酵液经固液分离得到菌丝体,菌丝体经乙醇抽提得FGFC1抽提液,采用AmberliteTM XAD-1600型树脂吸附抽提液中的部分色素与杂质,使用Uni PS30-300型色谱柱进一步去除色素与同系物杂质,得到FGFC1纯化液,调至pH 3沉淀并真空干燥,即得FGFC1固态粉末,其结构经质谱(MS)和核磁(NMR)初步验证。该法制备的FGFC1纯度为97%,总收率为48%。The separation and purification processes of fibrinolytic compound FGFC1 with high-purity from the fermentation broth were established.The Stachybotrys longispora FG216 mycelium was obtained by solid-liquid separation and the extract liquid containing FGFC 1 was got after ethanol extraction.AmberliteTM XAD-1600 resin column was used to remove part of pigments and impurities,and Uni PS30-300 chromatographic column was used to remove the rest pigments and homologues.Then the purified liquid of FGFC 1 was got.The FGFC 1 powder was finally obtained after precipitation at pH 3 and vacuum freeze-drying.Its chemical structure was identified by MS and NMR.The product purity was 97％ with a total yield of 48％.

关 键 词：FGFC1 促纤溶活性化合物 长孢葡萄穗霉 分离纯化 

分 类 号：R972[医药卫生—药品]