心钠素基因预防血管损伤后内膜增殖的实验研究  

Experimental study on ANF gene transfer for prevention of intima proliferation after artery injury

作　　者：杨春梅[1] 马根山[1] 黄峻[1] 潘世扬[2] 王连生[1] 李春坚[1] 黄佩珺[2] 

机构地区：[1]南京医科大学第一附属医院心脏科,210029 [2]南京医科大学第一附属医院临检中心,210029

出　　处：《江苏医药》2001年第4期243-245,共3页Jiangsu Medical Journal

基　　金：江苏省教委自然科学基金! (95 0 5 4)

摘　　要：目的　探讨心钠素基因能否预防血管损伤后的内膜增殖反应。方法　将心钠素基因转染入兔左颈总动脉血管损伤处。观察转染后不同时间心钠素基因的存在、表达及对颈总动脉的影响。结果　转染后 3、8、2 1天检测出局部血管壁有心钠素基因的持续存在和表达。与对照组比转染后 3天内膜平滑肌细胞减少 2 8% (P <0 0 5 ) ,8天新生内膜面积减少 5 2 % (P <0 0 1) ,2 1天减少2 8 6 % (P <0 0 5 )。血浆中心钠素的含量转染后有升高趋势 ,无统计意义。Objective To detect whether ANF gene was able to prevent intimal hyperplasia after carotid injury.Methods pcDNA3/ANF construct was transferred into the injured artery.The existence of ANF cDNA,the expression of ANF gene products and its effect on injury carotid were tested at different time after transfection.Results There were ANF mRNA expression and ANF gene existence at 3,8 days and 21 days after delivery of hANF cDNA.Intima smooth muscle cells(SMC) decreased 28% after transfection of 3 days ( P< 0 05).The neointimal area decreased 52% after transfection of 8 days ( P< 0 01),and decreased 8 6% after delivery of 21 days ( P< 0 05) compared with control group.The content of ANF had elevatory trend but no statistical singificance.Conclusion pcDNA3/ANF gene could prevent intimal hyperplasia after carotid injury in vivo.

关 键 词：心钠素 基因治疗 内膜增殖 血管损伤 预后 

分 类 号：R543[医药卫生—心血管疾病] R394[医药卫生—内科学]