5-ALA表面修饰TiO_2诱导的光动力疗法体外灭活HL60细胞实验研究  

An Experimental Study on the Inactivation Induced by TiO_2 Modified 5-ALA on Leukemic HL60 Cells in vitro

作　　者：林柏瀚 陈丽[2] 洪旭亮 肖化[1] 艾保全[1] 熊建文[1] 

机构地区：[1]华南师范大学物理与电信工程学院,广东广州510006 [2]广东工业大学物理与光电工程学院,广东广州510006

出　　处：《激光生物学报》2014年第2期97-102,126,共7页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(61072029);广东省自然科学基金项目(10151063101000025;S2012010010661);广州市科技计划项目(2014J410009)

摘　　要：利用表面修饰的方法制备了5-ALA表面修饰的TiO2(5-ALA/TiO2),并利用傅里叶红外光谱,拉曼光谱以及紫外-可见光吸收光谱(UV-Vis)对样品进行了表征。利用Cell Counting Kit-8(CCK-8)法检测研究5-ALA/TiO2对HL60细胞的灭活效应。结果显示,5-ALA/TiO2能够显著地抑制HL60细胞的生长,5-ALA/TiO2对HL60细胞的灭活效率要明显高于5-ALA以及TiO2,实验中5-ALA/TiO2的灭活效率达到77.9%,相衬显微镜细胞形态学无损观察表明,细胞凋亡开始于细胞膜的破裂。同时,激光显微拉曼光谱检测显示,TiO2纳米粒子能够进入细胞内部,与细胞发生相互作用。The 5-aminolevulinic-adsorbed TiO2 （ 5-ALA/TiO2 ） were synthesized by a surface modification method. ATR-FTIR,Raman imagine and ultraviolet-visible absorption spectrocopy（UV-Vis） were employed to study the features of 5-ALA/TiO2. Then the inactivation of 5-ALA/TiO2 on leukemia tumors was investigated by using Cell Counting Kit-8 （ CCK-8 ） assay. Furthermore, the morphology and apoptotic percentage of treated cells by 5-ALA/TiO2 were studied. The inactivation efficiency of 5-ALA/TiO2 was higher than 5-ALA and TiO2, indicating that the inactivation efficiency could be effectively enhanced by synthesizing 5-ALA and TiO2. The inactivation efficiency of HL60 cells was 77.9%. The study on the morphology of treated cells by phase contrast microscope showed that cells apoptosis start from the burstof film of cell. The micro Ramans spectroscopy test showed that TiO2 nanoparticles can get inside and interact with the HL60 cell.

关 键 词：光动力疗法(PDT) 二氧化钛(TiO2) 5-氨基乙酰丙酸(5-ALA) 相衬显微镜 

分 类 号：R318.51[医药卫生—生物医学工程]