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作 者:周洁[1,2] 邹琳[1,3] 李佳[3] 王晓[1] 刘伟[1] 方磊[1] 李强[1] 张芳[3] 张永清[3]
机构地区:[1]山东省分析测试中心,山东济南250014 [2]中国中医科学院中药资源中心道地药材国家重点实验室培养基地,北京100700 [3]山东中医药大学,山东济南250355
出 处:《中国中药杂志》2014年第13期2460-2463,共4页China Journal of Chinese Materia Medica
基 金:国家自然科学基金项目(81303161,81102749,21202094,81130070);山东省自然科学基金项目(ZR2013HQ037,ZR2012HQ020);山东省科学院科技发展计划项目[科基合字(2012)第16号];山东省海外高层次人才专项资金项目
摘 要:该文采用1∶500,1∶1 000,1∶2 000浓度的烟水处理白花丹参,研究其对白花丹参生物量和有效物质积累的影响,测量指标包括地下部分鲜重、地下部分干重、分根数、最大根粗、平均根粗、平均根长、脂溶性成分(二氢丹参酮、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA)和水溶性成分(迷迭香酸、丹酚酸B)含量。结果显示,1∶500和1∶1 000浓度的烟水处理显著提高白花丹参地下部分鲜重和干重,分别达到98.01%,44.32%和85.71%,28.57%。1∶500浓度的烟水处理显著提高白花丹参最大根粗(58.44%)和地下部分干重(85.71%)。烟水处理后白花丹参中丹参酮I和丹参酮ⅡA含量显著提高,二氢丹参酮、隐丹参酮以及水溶性成分的积累未表现出显著性差异。研究表明烟水处理能有效提高白花丹参生物量和有效物质的积累,为白花丹参绿色生态种植提供新的思路。To study the effect of plant-derived smoke water on the accumulation of biomass and active substance of Salvia miltiorrhia f. alba, seedlings of S. miltiorrhiza were treated with different concentrations of smoke water ( 1 : 500, 1 : 1 000, 1: 2 000). The fresh weight and dry weight of underground part, the number of split-root, maximum root diameter, average root diameter, average root length, the content of lipophilic components and water-soluble components were measured. Results showed that fresh weight and dry weight of underground part were respectively improved by 98.01% ,44. 32% and 85.71% ,28.57% with significant difference by smoke water treatment with concentration of 1 : 500 and 1 : 1 000. Maximum root diameter and dry weight of underground part were re- spectively enhanced by 58.44% and 85.71% by smoke water with concentration of 1 : 500. The content of tanshinone Ⅰ and tanshinone Ⅱ s were improved by smoke water treatment, however there were no significantly difference on the content of cryptotanshinone and dihydrotan shinone. This study indicates that smoke water treatment could be used to improve the accumulation of biomass and active substance content of S. miltiorrhiza f. alba, which could provide new ideas for its green cultivating.
分 类 号:S567.5[农业科学—中草药栽培]
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