Box-Benhnken设计效应面法优选黄芪切制工艺  

Optmization for cutting procedure of Astragali Radix with Box-Behnken design and response surface method

作　　者：沈秀娟[1] 周倩[2] 孙立立[2] 戴衍朋[2] 闫雪生[2] 

机构地区：[1]山东中医药大学,山东济南250355 [2]山东省中医药研究院,山东济南250014

出　　处：《中国中药杂志》2014年第13期2498-2503,共6页China Journal of Chinese Materia Medica

基　　金：中医药行业科研专项(20110700705);山东省科技发展计划项目(2011-170);国家中医药管理局全国名老中医药专家传承工作室建设项目

摘　　要：黄芪始载于《神农本草经》,列为上品,为临床常用中药,切片多生用或蜜炙后应用,目前开展的研究中,对蜜炙工艺研究报道较多,而对黄芪切制工艺则缺少相关的研究报道,黄芪切制目前多凭药工经验操作,没有具体的切制工艺参数,很容易出现有效成分流失、发生霉变等多种问题,导致目前市场上流通的黄芪饮片质量良莠不齐,饮片和制剂的质量难以控制,严重影响了临床疗效,针对上述问题,该实验以优选黄芪的最佳切制工艺为研究目的,采用响应面分析法中的BoxBenhnken中心组合设计,以黄芪甲苷、毛蕊异黄酮苷含量和外观性状为考察指标,选择软化润制时间、干燥温度和干燥时间3个因素对黄芪切制工艺进行优选,得到了实验室最佳切制工艺参数:润制时间3 h,干燥温度50℃,干燥时间4 h。验证试验表明,确定的黄芪切制工艺稳定可行,对于规范黄芪切制工艺有一定的意义。Astragali Radix was firstly recorded in the ＂Shen Nong＇s Herbal Classic＂ as a top-grade and commonly used tradi- tional Chinese medicine. Its frequently used slices include raw Astragali Radix and honey-processed products. In current studies, many reports were made on honey-processed Astragali Radix, whereas fewer study reports were made on the cutting process of Astragali Ra- dix. Currently, because Astragali Radix is primarily cut by drug workers according to their operating experience, but with out specific cutting parameters, it is easy to cause the loss or mildew of active ingredients. As a result, the quality of Astragali Radix circulated in the market is not guaranteed, and the quality of their slices and preparations are hard to be controlled, which seriously impact the clini- cal efficacy. In response, this experiment was performed, in which the optimum cutting process of Astragali Radix was taken as the study objective, the Box-Benhnken central composite design in the response surface analysis was adopted, and the content and appear- ance character of astragaloside and calycosin-7-glucoside were regarded as the study indicators. Three factors, namely the softening time, the drying temperature and the drying time, were selected to optimize the cutting process of Astragali Radix and obtain the optimum cutting process parameters as follows： the softening time was 3 hours, the drying temperature was 50℃, and the drying time was 4 hours. According to the verification test, the Astragali Radix cutting process is steady and feasible, which has certain significance for normalizing the cutting process of Astragali Radix.

关 键 词：黄芪 Box-Benhnken设计 响应面法 切制工艺 黄芪甲苷 毛蕊异黄酮苷 

分 类 号：R283[医药卫生—中药学]