高效液相质谱联用技术鉴定刺五加注射液中苯丙素类化学成分  

Identification of phenylpropanoids in Ciwujia injection by HPLC-MS

作　　者：黄婧[1] 邵青[1] 项彦华 葛志伟[1] 范骁辉[1] 

机构地区：[1]浙江大学中药科学与工程学系,浙江杭州310058 [2]黑龙江珍宝岛药业股份有限公司,黑龙江哈尔滨150060

出　　处：《中国中药杂志》2014年第13期2513-2520,共8页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2012CB518405)

摘　　要：刺五加药材的化学组成已有报道,而未见刺五加注射液化学物质基础的研究报道。该研究采用液相色谱离子阱质谱(LC-MSn)和液相色谱四极杆飞行时间质谱(LC-Q-TOF-MS)技术对刺五加注射液中苯丙素类化学成分进行定性分析。采用UltmateTMXB-C18色谱柱(4.6 mm×250 mm,5μm),以0.5%甲酸溶液和乙腈为流动相进行梯度洗脱,流速0.8 mL·min-1,柱温20℃。根据高分辨和多级质谱数据,并结合对照品及文献数据,在刺五加注射液中共推断出54个苯丙素类成分,包括苯丙酸类成分34个,木脂素类成分16个,香豆素类成分4个,其中28个成分在刺五加中未见报道,通过对照品比对鉴定了14个化合物结构。该研究建立的方法可以简便快速地对刺五加注射液中的苯丙素类化学成分进行鉴定,研究结果为明确刺五加注射液化学物质基础提供了科学数据。There are reports about the chemical compounds of Ciwujia herbs, but with no study report about the chemical ma- terial basis of Ciwujia injection （CWJI）. In this study, LC-MSa and LC-Q-TOF-MS techniques were adopted for a qualitative analysis on phenylpropanoids in CWJI. The UltmateTM XB-CI8 column （4. 6 mm × 250mm, 5μm） was adopted and eluted with the mobile phase of 0. 5% formic acid-water and acetonitrile, with the flow rate at 0. 8 mL·min-1 and the column temperature at 20℃. Based on the data of high-resolution and multi-stage MS, control products and literatures, altogether 54 phenylpropanoids were identified in Ci- wujia Injection, including 34 phenylpropanoids, 16 ligans and 4 coumarins. Among them, 28 were reported for the first time in Ciwu- jia, and 14 compound structures were identified in comparison with the control products. The method established in this study could be used to simply and rapidly identify phenylpropanoids in CWJI. The findings provide scientific data for defining the chemical material basis of CWJI.

关 键 词：刺五加注射液 LC-MS LC-Q-TOF-MS 苯丙素类成分 

分 类 号：R284.1[医药卫生—中药学] O657.63[医药卫生—中医学]