脑缺氧缺血致新生大鼠脑皮质、海马组织中c-fos基因的表达  

C-fos induction in cortex,hippocampus after neonatal hypoxia ischemia

作　　者：丁艳洁[1] 汤云珍[1] 蒋犁[1] 李海浪[1] 

机构地区：[1]东南大学附属中大医院儿科,南京210009

出　　处：《实用儿科临床杂志》2001年第2期66-68,共3页Journal of Applied Clinical Pediatrics

基　　金：铁道部科技基金资助项目!(项目编号 :97-J-2 4)

摘　　要：目的　研究缺氧缺血后新生大鼠脑皮质、海马组织中c fos基因的表达。方法　利用新生大鼠脑缺氧缺血模型 ,采用RT PCR技术与免疫组化方法 ,观察缺氧缺血后皮质、海马组织中即早基因c fosmRNA及c fos蛋白的表达。结果　与对照组比较 ,实验组缺氧缺血后 0～ 1h ,结扎侧皮质与海马c fos基因的mRNA表达增加 ,2h后逐渐回至对照水平。缺氧缺血后 2～ 4h结扎侧皮质与海马的阳性细胞率明显升高 ,与对照组相比有显著性差异 (P <0 .0 5 )。与c fosmRNA的表达高峰相比 ,c fos蛋白的表达延迟 1～ 2h。结论　c fos基因表达上调可能参与诱发缺氧缺血后皮质、海马脑损伤。Objective To explore the expression of c fos gene in neonatal rat cortex, hippocampus after hypoxia ischemia.Methods RT PCR and immunohistochemisty were used to detect the expression of c fos mRNA and c fos protein in cortex, hippocampus of hypoxia ischemia neonatal rats.Results Compared with controls, c fos mRNA transiently increased at 0～1 h post hypoxia in the ligated side cortex and hippocampus, then returned to the level in controls after 2 h. By immunohistochemistry, the positive rate of c fos protein increased to the high level after 2 hours post hypoxia in ligated side (P<0.05), and the peak time of it delayed about 1h.Conclusion The transient expression increase of c fos gene might induce hypoxia ischemia damage in the cortex and hippocampus.

关 键 词：脑缺氧 脑缺血 新生大鼠 FOS基因 脑皮质 海马组织 

分 类 号：R722.12[医药卫生—儿科]