胆固醇缺乏对Jurkat T淋巴细胞膜脂流动性的影响  

Effect of cholesterol deficiency on the membrane fluidity of Jurkat T lymphocytes

作　　者：周东明[1] 糜漫天[1] 郎海滨[1] 杨镇洲[1] 

机构地区：[1]第三军医大学营养卫生学教研室,重庆400038

出　　处：《卫生研究》2001年第2期83-85,共3页Journal of Hygiene Research

摘　　要：:用荧光偏振技术、3H - Td R掺入法及流式细胞术等方法 ,从细胞膜脂流动性这一角度来探讨胆固醇缺乏抑制 Jurkat细胞增殖的机制 ,阐明胆固醇对维持淋巴细胞正常功能的重要性。结果显示 :经去脂血清培养基培养的 Jurkat细胞加 lovastatin(合成胆固醇的限速酶的抑制剂 )处理 3天后 ,细胞膜脂流动性明显增大 ,3H- Td R掺入率明显下降 ,细胞增殖受抑制 ,细胞受阻于 G0 / G1 期 ,加入一定量的低密度脂蛋白可以缓解上述变化。结果提示 ,无论是内源性还是外源性缺乏胆固醇都能使 Jurkat细胞膜脂流动性发生变化 ,推测这一变化与细胞 G0 / G1 期阻滞及细胞增殖受抑制有关。The mechanism of cholesterol deficiency on inhibiting the proliferation of T lymphocytes was investigated on cell membrane fluidity by using fluorescence polarization measurement, 3H TdR incorporation test and flow cytometer analysis in Jurkat cells, in order to clarify the importance of cholesterol in maintaining the normal function of lymphocytes.The results showed that cell membrane fluidity was increased,cell proliferation was inhibited and blocked on G 0/G 1 phase in Jurkat cells after being cultured with lovastatin (an inhibitor of rate limiting enzyme for cholesterol synthesis)for 3 days. When the cells were treated with LDL. The changes could be partially buffered. These results suggest that the change of membrane fluidity may be caused by cholesterol deficiency on the proliferation of Jurkat cells.

关 键 词：胆固醇缺乏 JURKAT细胞 膜流动性 细胞周期 T淋巴细胞 

分 类 号：Q548.1[生物学—生物化学] Q591.5