救脑宁注射液对缺氧/缺糖神经细胞凋亡的影响  

Effects of Jiunaoning Injection on Neuron Apoptosis Induced by Hypoxia and Hypoglycemia

作　　者：何丽云[1] 孙塑伦[1] 范吉平[1] 黄启福[1] 王硕仁[1] 朱陵群[1] 张壮[1] 

机构地区：[1]北京中医药大学东直门医院,北京100700

出　　处：《北京中医药大学学报》2001年第2期21-23,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金："九五"国家科技攻关!课题 (No .96_90 6_0 6_0 1)

摘　　要：研究救脑宁注射液对缺氧 /缺糖致神经细胞凋亡的影响。采用人神经母细胞瘤细胞株 (SH SY5H) ,建立缺氧 /缺糖诱导凋亡模型 ,以流式细胞技术和活细胞荧光双染色法观察凋亡的发生及救脑宁注射液抑制细胞凋亡的作用。结果 :缺氧 /缺糖培养能诱导神经细胞凋亡 ,流式细胞仪检测 ,在G1峰前出现显著的凋亡峰 ,凋亡率 42 99%± 7 73%。荧光双染色后 ,荧光显微镜下观察到凋亡时细胞核染色质凝聚。中大剂量救脑宁注射液能降低细胞凋亡率 ,与模型组相比差异显著 (P<0 0 1 ) ,与神经生长因子组相比差异无统计学意义。结论 :救脑宁注射液可抑制缺氧 /缺糖诱导的神经细胞凋亡 ,具有神经细胞保护作用。The effects of Jiunaoning Injection (JI) on neuron apoptosis induced by hypoxia and hypoglycemia were studied. SH SY5Y was used to establish the model of neuron apoptosis induced by hypoxia and hypoglycemia. Flow cytometry and living cell fluorescent double staining were used to observe the neuron apoptosis and the inhibitory effects of JI on the neuron apoptosis. The results showed that neuron apoptosis could be induced by the hypoxic and hypoglycemic culture, and an obvious apoptotic peak in front of the G1 peak with an apoptotic ratio of 42 99%±7 73% was found by the flow cytometry analysis; after the fluorescent double staining was performed, the coacervation of cytoplasm in the apoptotic neurons was observed by the fluorescence microscope. The results also showed that a medium or a large dose of JI could decrease the apoptotic ratio of the neuron in the JI treating group; and there was a significant difference between the result from the JI treating group and that from the model group ( P <0 01), and an insignificant difference between the result from the JI treating group and that from the nerve growth factor group ( P >0 05). Therefore, it could be concluded that JI can inhibit the neuron apoptosis induced by hypoxia and hypoglycemia, and possesses protective effects on the neuron.

关 键 词：救脑宁注射液 缺氧 缺糖 神经细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]