Optimization of the Method to Obtain Effective Sterile Explants in Tissue Culture of Ardisia mamillata Hance  

虎舌红组织培养中有效无菌材料的获得研究(英文)

作　　者：曾云英[1,2] 

机构地区：[1]江苏开放大学 [2]江苏城市职业学院,江苏南京210036

出　　处：《Agricultural Science & Technology》2014年第6期916-917,936,共3页农业科学与技术（英文版）

基　　金：Supported by the Research Funds of Jiangsu Open University/the City Vocational College of Jiangsu during the 12th Five-Year Plan Period(12SEW-Y-026)~~

摘　　要：In the present study, we optimized the procedures to pretreat the parental material, to collect, sterilize and inoculate the explants in the tissue culture of Ar- disia mamillata Hance, so that more effective sterile explants can be obtained. The results revealed the optimal procedures. In detail, the parental materials were trans- ferred and pretreated in laboratory in February. The stem tips of new branches were collected in middle March, cleaned with eradicator and then rinsed with tap water for 1-2 h. After that, the explants were surface sterilized with 70% alcohol for 30 s, rinsed with sterile water 2-3 times, sterilized with 0.1% mercuric chloride for 4 min, rinsed with sterile water three times and sterilized with 0.1% mercuric chlo- ride for 4 min again. Finally, they were inoculated into medium after being rinsed with sterile water 5-8 times. By this method, the pollution rate of explants can be greatly reduced while their survival rate can be significantly improved.该研究以虎舌红为试材,探讨外植体母本的预处理、外植体的采集、灭菌、接种等处理方式对获得有效无菌材料的途径和方法的影响。结果表明：2月将母株移入室内,进行预处理,3月中旬取当年生枝条和萌蘖条上的茎尖,用去污剂（肥皂水或洗衣粉水）浸泡并刷洗后,流水冲洗1~2 h,用75%的酒精表面消毒30 s,无菌水冲洗2~3次后放入0.1%升汞中灭菌,先用升汞消毒4 min,无菌水冲洗3次,再用升汞消毒4 min,无菌水冲洗5~8次后进行有效接种,这种方法能有效降低污染率,提高成活率。

关 键 词：Tissue culture Sterile material Ardisia mamillata Hance 

分 类 号：Q943[生物学—植物学]