乳酸乳球菌在疫苗递呈载体中的应用  

New Applicntion in Lactococcus Lactis as Vaccine Delivery Systems

作　　者：刘晓锐[1] 张晓红[2] 吴洁[1] 

机构地区：[1]中国药科大学生命科学与技术学院微基因药学实验室,江苏南京210009 [2]南京医科大学南京市妇幼保健院生殖医学国家重点实验室,江苏南京210004

出　　处：《药物生物技术》2014年第1期75-80,共6页Pharmaceutical Biotechnology

基　　金：国家自然科学基金资助项目(No.81172973;31270985);中央高校基本科研业务费研究生专项(No.JKY2011076)

摘　　要：乳酸菌是人和动物肠道内的常见细菌,被公认为安全级微生物。近年来,对于乳酸菌作为宿主菌表达外源蛋白或抗原的研究取得了一定进展。乳酸乳球菌(Lactococcus lactis)是乳酸菌的模式菌种,其基因组测序已经完成,因而方便研究者根据实际需要利用分子生物学工具将其改造为各种表达载体可用的受体菌。目前已经开发了一系列质粒型克隆载体和整合型克隆载体系统及蛋白定位系统,已有多种病原微生物抗原成功在乳酸乳球菌中表达,重组乳酸乳球菌作为粘膜免疫疫苗的递呈载体呈现非常好的应用前景,文章介绍了近年来乳酸乳球菌在疫苗递呈载体中的应用情况,重点阐述了其在抗感染、抗肿瘤、抗自身免疫性方面的研究进展及其在疫苗研制中遇到的困难及需要解决的问题,并对其应用前景进行了展望。Lactic acid bacteria are normal in human and animal intestinal which are generally recognized as safe microorganism. Lactococcus lactis is the mode bacteria of lactic acid bacteria and its genome has been sequenced, and using the molecular biology tools,it＇s easy for the modification of recipient bacteria which are suitable for all kinds of expression vector. Currently it has been developed that a series of plasmid cloning vector,integrated cloning vector system and protein localization system, meanwhile a variety of pathogenic microbial antigens have successfully expressed in Lactococcus lactis. The application situation of Lactococcus lactis in vaccines presenting carrier in recent years have been described, and elaborating research progress in the anti-infective, anti-cancer, anti-autoimmune aspects and difficulties encountered in vaccine development and the prospect are well explained.

关 键 词：乳酸乳球菌 疫苗 抗原 表达 粘膜免疫 载体 

分 类 号：Q812[生物学—生物工程]