从调节性T细胞和Th17细胞变化探讨抗敏平喘方治疗哮喘机制  

Study on mechanisms of anti-asthma by Kangmin Pingchuan Formula via balance of regulatory T cells and Th17 cells

作　　者：严展鹏[1] 张晓震[2,3] 徐东宁[2,3] 叶娟[2,3] 朱晓静[2,3] 滕之颖 曹鹏[2,3] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210023 [2]江苏省中医药研究院,江苏南京210028 [3]中国中医科学院江苏分院,江苏南京210028

出　　处：《中草药》2014年第12期1731-1735,共5页Chinese Traditional and Herbal Drugs

基　　金：江苏省中医药领军人才培养基金(RC1006)

摘　　要：目的从调节性T细胞（Tregs）和Th17细胞亚群平衡方面来探讨抗敏平喘方治疗哮喘的机制。方法将6~8周龄雌性BALB/c小鼠随机分为5组：对照组、模型组、泼尼松（5.5 mg/kg）组、抗敏平喘方低和高剂量（4.95、9.90 g/kg）组;用卵清蛋白诱导小鼠哮喘模型,每天雾化后ig给药,连续15 d,末次给药后处死小鼠,通过病理切片HE染色观察肺组织的损伤情况,用流式细胞术测定血液中Th17和Tregs细胞亚群的变化比例;ELISA法检测小鼠血清中白细胞介素-4（IL-4）、转化生长因子-β（TGF-β）和肿瘤坏死因子-α（TNF-α）蛋白的表达水平。结果病理切片HE染色显示抗敏平喘方能够改善哮喘小鼠肺组织损伤,降低淋巴细胞对肺组织的浸润水平;抗敏平喘方能够下调血液中Th17细胞比例和上调Tregs细胞比例,并且存在剂量依赖效应;抗敏平喘方同时能够显著提高血液中IL-4和TGF-β水平并降低TNF-α水平。结论抗敏平喘方能够通过影响Th17细胞和Tregs在血液中的比例以及相关炎症因子的水平而达到治疗哮喘的作用。Objective To study the possible mechanisms of Chinese materia medica Kangmin Pingchuan Formula via regulating the balance of regulatory T cells （Tregs） and Thl7 cells. Methods The female BALB/c mice were divided into five groups： control, model, Prednisone （5.5 mg/kg）, Kangmin Pingchuan Formula low- （4.95 g/kg） and high-dose （9.90 g/kg） groups; Ovalbumin was used to make the asthmatic model of mice, the drug was ig administered daily after atomization for consecutive 15 d, then the mice were killed after the last administration. The paraffin-embedded tissue sections of the lungs in mice were stained by HE; Thl 7 cells and Tregs in blood were detected by flow cytometry; IL-4, TGF-13, and TNF-ct in serum of mice were detected by ELISA assay. Results HE staining showed that Kangmin Pingchuan Formula could alleviate the inflammation in the lung tissue of mice, decrease the proportion of Thl7 cells, increase the proportion of Tregs in blood, promote TGF-13 levels, and inhibit TNF-ct levels in blood. Conclusion Kangmin Pingchuan Formula could treat the asthma through impacting the proportion of Thl7 cells and Tregs in blood as well as the levels of related inflammatory cytokines in mice.

关 键 词：哮喘 抗敏平喘方 调节性T细胞 Th17细胞 炎症细胞因子 

分 类 号：R285.5[医药卫生—中药学]