毛竹IDD基因家族的生物信息学分析  

Bioinformatic Aanlysis of IDD Gene Family in Phyllostachys heterocycla

作　　者：关鹰[1] 许在恩 郭小勤[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300

出　　处：《核农学报》2014年第6期998-1005,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(30901155);"973"项目(2012CB723008);浙江省自然科学基金(Y307499)

摘　　要：IDD基因家族编码一种混合型的转录因子,本文通过本地Blast从毛竹（Phyllostachys heterocycla）基因组数据库获取到了9个IDD家族基因,并命名为PhID1和PhIDD1-8,其氨基酸序列均具IDDdomain结构特征,一个假定的核定位信号、2个C2H2和2个C2HC。氨基酸理化性质分析发现,PhIDD蛋白均为亲水性蛋白,含量较多是丙氨酸（Ala）、丝氨酸（Ser）和甘氨酸（Gly）,全序列等电点分布在8.8-9.7之间,仅PhIDD2蛋白的等电点为5.6。除PhIDD2外,IDD-domain的等电点与全序列等电点基本一致。PhIDD蛋白二级结构含量分析发现无规则卷曲和α-螺旋含量最多,β-转角和延伸链分布在整个蛋白中。蛋白磷酸化位点预测结果显示磷酸化位点数量在20-29个不等。三维结构分析显示,PhIDD1在63-175氨基酸处,会形成α-螺旋,β-折叠及无规则卷曲及明显的锌原子结合位点。IDD gene family encode hybrid transcription factor. In this study, nine IDD genes in Phyllostachys heterocycla, named PhlDD1 and PhlDD1 - 8 were obtained by local blast. They all contain IDD-domain which is a putative nuclear location signal and four zinc finger motifs. All nine putative proteins were hydrophilie, and their isoelectric point distributed between 8.8 and 9.7 except for PhlDD2 （5.6）. Ala, Ser and Gly were abundant in these proteins. Constructions of α - helixs and random coils in these proteins were dominant elements, β - turns and extended strains interspersing over the whole proteins. The prediction of these proteins showed that the number of phosphorylation sites were from 20 to 29, α -helixs, β -turns and random coils of PhIDD1 were formed at 63 - 175amino acid and there is a zinc binding site at this space.

关 键 词：毛竹 IDD基因家族 转录因子 生物信息学 

分 类 号：S795.7[农业科学—林木遗传育种]