Cloning,Localization and Expression Analysis of ZmHsf-like Gene in Zea mays  

作　　者：LI Hui-cong LI Guo-liang LIU Zi-hui ZHANG Hong-mei ZHANG Yan-min GUO Xiu-lin 

机构地区：[1]Institute of Genetics and Physiology Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province

出　　处：《Journal of Integrative Agriculture》2014年第6期1230-1238,共9页农业科学学报（英文版）

基　　金：supported by grants from the Key Project of Application Basic Research of Hebei Province,China (12965517D);the Youth Fund of Hebei Academy of Agriculture and Forestry Sciences,China (A09110103).

摘　　要：Using homology cloning method, a heat shock transcription factor （Hsf） like gene, ZmHsf-like, was cloned from maize （Zea mays） leaves. Sequence analyses showed that the open reading frame （ORF） of the gene ZmHsf-like is 1 404 bp long, encoding 467 amino acids. The sequence of amino acids encoded by ZmHsf-like contains the most conserved and typical DNA-binding domain of Hsf family. By bombardment into onion epidermis, we ifrstly found that the ZmHsf-like was subcellular-located in nucleus. NucPred analysis revealed there is a classic NLS of KKRR peptide in protein. Real-time PCR showed that ZmHsf-like gene expressed in leaves, stems and roots of maize seedlings under normal growth conditions, and the highest expression level was in roots, lower in leaves and the lowest in stems. The ZmHsf-like gene expression could be up-regulated by heat shock, PEG, ABA, and H2O2 in different degrees, among which the heat shock and ABA worked more efifciently. Obvious differences of the peak value and its corresponding time point of ZmHsf-like gene expression were observed among treatments. Experiments with inhibitor further suggested that the up-regulation ZmHsf-like gene expression of heat shock was H2O2-dependent while the induction of ZmHsf-like with PEG did not depend on the existence of H2O2. These results pointed out that ZmHsf-like gene probably regulates responsive reactions to abiotic stresses especially heat shock and drought through different signal transduction pathways.Using homology cloning method, a heat shock transcription factor （Hsf） like gene, ZmHsf-like, was cloned from maize （Zea mays） leaves. Sequence analyses showed that the open reading frame （ORF） of the gene ZmHsf-like is 1 404 bp long, encoding 467 amino acids. The sequence of amino acids encoded by ZmHsf-like contains the most conserved and typical DNA-binding domain of Hsf family. By bombardment into onion epidermis, we ifrstly found that the ZmHsf-like was subcellular-located in nucleus. NucPred analysis revealed there is a classic NLS of KKRR peptide in protein. Real-time PCR showed that ZmHsf-like gene expressed in leaves, stems and roots of maize seedlings under normal growth conditions, and the highest expression level was in roots, lower in leaves and the lowest in stems. The ZmHsf-like gene expression could be up-regulated by heat shock, PEG, ABA, and H2O2 in different degrees, among which the heat shock and ABA worked more efifciently. Obvious differences of the peak value and its corresponding time point of ZmHsf-like gene expression were observed among treatments. Experiments with inhibitor further suggested that the up-regulation ZmHsf-like gene expression of heat shock was H2O2-dependent while the induction of ZmHsf-like with PEG did not depend on the existence of H2O2. These results pointed out that ZmHsf-like gene probably regulates responsive reactions to abiotic stresses especially heat shock and drought through different signal transduction pathways.

关 键 词：MAIZE HSF ZmHsf-like stress nuclear-localization 

分 类 号：S513[农业科学—作物学]