单个大鼠胎肝干细胞的激光光镊喇曼光谱  

Laser-tweezer Raman Spectroscopy Study of Single Rat Fetal Liver Stem Cell

作　　者：王雁军[1] 覃宗定[2] 姚辉路 汪蕴[1] 丰美福[1] 

机构地区：[1]中国科学院动物研究所生物膜与膜生物工程国家重点实验室,北京100101 [2]广西科学院生物物理实验室,南宁530003

出　　处：《光子学报》2014年第6期106-110,共5页Acta Photonica Sinica

基　　金：国家自然科学基金(No.60868002)资助

摘　　要：激光光镊喇曼光谱系统是将激光光镊与喇曼光谱相结合的一项光学技术,可以在接近自然的生理状态下研究单个生物细胞的识别及其构成成分的检测.本文采用胶原酶消化法,分离10~21天不同孕期大鼠的胎肝干细胞和成体大鼠的肝实质细胞,应用激光光镊喇曼光谱技术检测分离到单个胎肝干细胞和肝实质细胞,以及WB-F344大鼠肝干细胞系细胞,并比较了胎肝干细胞和肝实质细胞的喇曼光谱.结果发现,13天以前孕大鼠胎肝干细胞在1 336cm-1（代表多聚核苷酸链（嘌呤））和1 446cm-1（代表蛋白的弯曲模型）处的峰依次增高,13天孕大鼠胎肝干细胞在1 336cm-1和1 446cm-1处的平均峰值最高,14天以后逐渐降低.结果表明激光光镊喇曼光谱系统可以鉴别不同孕期的胎肝细胞和成体肝实质细胞,分析胎肝干细胞的分化机理,为临床应用肝干细胞治疗奠立基础.Laser tweezers Raman spectroscopy system is an optical technique combining the laser tweezers and Raman spectroscopy technique, which could be able to study recognition and detect constituent components of single biological cell in close to its natural state. Rat fetal liver stem cells at 10~21 days of gestation and adult rat hepatocytes were separated by collagenase digestion. Single fetal liver stem cell, hepatocyte and WB-F344 rat liver stern cell line were detected by laser tweezers Raman spectroscopy technique, and their Raman spectrum was compared. It was indicated that the value of 1 336 cm （assigned to Polynucleotide chain, DNA-purine bases） and 1 446 cm^-1 （CH2 bending mode of proteins） band increased before 13th day and decreased after 14th day of gestation, and the mean of peak value of 1 336 cm^-1 and 1 446 cm^-1 bands at 13th day of gestation was the highest. The results showed that the Laser tweezers Raman spectroscopy system could differentiate fetal liver stem cells of different period of gestation from adult hepatocytes, and analyze differentiation and development mechanism of fetal liver stem cells, which lay a foundation for clinical application of hepatic stem cells therapy.

关 键 词：光谱学 细胞生物物理学 激光光镊喇曼光谱 大鼠胎肝干细胞 喇曼光谱 激光束 细胞 分析 

分 类 号：O657.3[理学—分析化学]